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Purified Mouse Anti-Heme Oxygenase 1

BD Transduction Laboratories™ Purified Mouse Anti-Heme Oxygenase 1

Clone 23/Heme Oxygenase 1

Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human Heme Oxygenase 1 (HO-1) aa. 150-286
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
32 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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Antibody Details
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23/Heme Oxygenase 1

The 23/Heme Oxygenase 1 monoclonal antibody specifically recognizes Heme oxygenase 1 (HO-1), also known as Heat Shock Protein 32 (HSP32). HO-1 is a 32 kDa monooxygenase encoded by HMOX1. Heme oxygenases 1 and 2 (HO-1, HO-2) cleave the heme molecule, resulting in the production of carbon monoxide (CO) and biliverdin. While HO-2 is constitutively expressed in tissues, HO-1 is rapidly induced by several stimuli such as lutathione depletion, hemin, heat shock, heavy metals, oxidative stress, oxidized LDL, anoxia, and endotoxic shock. HO-1 and its byproducts have potent antioxidant, antiproliferative, anti-inflammatory and anti-apoptotic effects in various cell types. In humans, HO-1 deficiency is associated with susceptibility to oxidative stress, increased inflammation and endothelial cell damage. In immune cells, HO-1 is required for the activation of interferon (IFN) regulatory factor 3 (IRF3) after Toll-like receptor 3 or 4 stimulation or viral infection. It is also upregulated on T cells upon TCR stimulation.

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Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
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Citations & References
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Development References (4)

  1. Foresti R, Clark JE, Green CJ, Motterlini R. Thiol compounds interact with nitric oxide in regulating heme oxygenase-1 induction in endothelial cells. Involvement of superoxide and peroxynitrite anions. J Biol Chem. 1997; 272(29):18411-18417. (Biology). View Reference
  2. Ishikawa K, Navab M, Leitinger N, Fogelman AM, Lusis AJ. Induction of heme oxygenase-1 inhibits the monocyte transmigration induced by mildly oxidized LDL. J Clin Invest. 1997; 100(5):1209-1216. (Biology). View Reference
  3. Suzuki M, Ishizaka N, Tsukamoto K. Pressurization facilitates adenovirus-mediated gene transfer into vein graft. FEBS Lett. 2000; 470(3):370-374. (Clone-specific: Western blot). View Reference
  4. Yoshida T, Biro P, Cohen T, Muller RM, Shibahara S. Human heme oxygenase cDNA and induction of its mRNA by hemin. Eur J Biochem. 1988; 171(3):457-461. (Biology). View Reference
View All (4) View Less
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.