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BD Transduction Laboratories™ Purified Mouse Anti-Fyn
Clone 25/FYN (RUO)
Western blot analysis of fyn on Jurkat cell lysate. Lane 1: 1:250, lane 2: 1: 500, lane 3: 1:1000 dilution of anti-fyn.
Immunofluorescent staining of A431 cells with anti-fyn antibody.
Western blot analysis of fyn on Jurkat cell lysate. Lane 1: 1:250, lane 2: 1: 500, lane 3: 1:1000 dilution of anti-fyn.
Immunofluorescent staining of A431 cells with anti-fyn antibody.
Product Details
BD Transduction Laboratories™
Human (QC Testing), Rat, Dog (Tested in Development)
Mouse IgG2b
Human fyn aa. 1-132
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
59 kDa
250 µg/ml
AB_397565
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610163 Rev. 1
Antibody Details
25/FYN
Stimulation of the T cell antigen receptor (TcR) results in protein tyrosine phosphorylation via non-receptor tyrosine kinases. The src-related p59[fyn] kinase is one such enzyme that associates with the TcR. Ligation of the TcR activates the protein kinase activity of p59[fyn] in various human T cells. Fyn interacts with the CD3-ζ chains through its N-terminal region. In turn, fyn binds other proteins through its SH2 and SH3 domains. These proteins (p82 and p116) may serve as substrates and/or mediators of fyn activity. A 72 kDa fyn-related protein binds to the TcR/CD3 complex in certain T cells. This protein, known as p72[fyn-R], is a variant of the fyn kinase and contains at least four novel Ser/Thr phosphorylation sites. These sites may make p72[fyn-R] less susceptible to the action of phosphatases than p59[fyn]. Like p59[fyn], p72[fyn-R] is constitutively associated with its receptor. The exact relationship between these two proteins is not yet clear. However, p72[fyn-R] immunoprecipitates with various antisera raised to either the N- or C-terminus of fyn. Similar to src and yes, fyn is highly expressed in brain. The localization of fyn in mouse brain and its interaction with myelin-associated glycoprotein (MAG) suggests that the fyn kinase has a role in the sensory network and in myelination during early stages of CNS formation.
610163 Rev. 1
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610163 Rev.1
Citations & References
View product citations for antibody "610163" on CiteAb
Development References (4)
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Derkinderen P, Toutant M, Kadare G, Ledent C, Parmentier M, Girault JA. Dual role of Fyn in the regulation of FAK+6,7 by cannabinoids in hippocampus. J Biol Chem. 2001; 276(41):38289-38296. (Clone-specific: Immunoprecipitation, Western blot). View Reference
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Feuillet V, Semichon M, Restouin A. The distinct capacity of Fyn and Lck to phosphorylate Sam68 in T cells is essentially governed by SH3/SH2-catalytic domain linker interactions. Oncogene. 2002; 21(47):7205-7213. (Clone-specific: Immunofluorescence, Western blot). View Reference
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Kawakami T, Kawakami Y, Aaronson SA, Robbins KC. Acquisition of transforming properties by FYN, a normal SRC-related human gene. 1988; 85(11):3870-3874. (Biology). View Reference
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Zeng L, Si X, Yu WP, et al. PTP alpha regulates integrin-stimulated FAK autophosphorylation and cytoskeletal rearrangement in cell spreading and migration. J Cell Biol. 2003; 160(1):137-146. (Clone-specific: Immunoprecipitation, Western blot). View Reference
610163 Rev. 1
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.