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Purified Mouse Anti-Connexin-43
Purified Mouse Anti-Connexin-43
Western blot analysis of Connexin-43 on a rat cerebrum lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- Connexin-43 antibody.
Purified Mouse Anti-Connexin-43

Immunofluorescence staining on WI38 cells (human lung).

Western blot analysis of Connexin-43 on a rat cerebrum lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- Connexin-43 antibody.

Immunofluorescence staining on WI38 cells (human lung).

Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Dog, Chicken (Tested in Development)
Mouse IgG1
Rat Connexin-43 aa. 252-270
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required), Immunoprecipitation (Not Recommended)
43 kDa
250 µg/ml
AB_397473
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610062 Rev. 1
Antibody Details
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2/Connexin-43

Gap junctions are intercellular protein pores. They enable cell-to-cell communication by allowing passage of ions and other small molecules. The subunits of gap junction channels are assembled from a family of proteins called connexins. Individual connexin molecules join to make hexameric hemichannels termed connexons; these structures dock to connexons on neighboring cells, forming gap junction pores. Connexin-43 is a member of the connexin family possessing four transmembrane regions, with cytoplasmic amino and carboxyl terminals. It undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. In addition, it is believed that phosphorylation of connexin-43 plays a regulatory role both in the assembly of connexons and in gating activity at the gap junction.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610062 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610062 Rev.1
Citations & References
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Development References (5)

  1. Giepmans BN, Hengeveld T, Postma FR, Moolenaar WH. Interaction of c-Src with gap junction protein connexin-43. Role in the regulation of cell-cell communication. J Biol Chem. 2001; 276(11):8544-8549. (Biology: Immunoprecipitation, Western blot). View Reference
  2. Laird DW, Castillo M, Kasprzak L. Gap junction turnover, intracellular trafficking, and phosphorylation of connexin43 in brefeldin A-treated rat mammary tumor cells. J Cell Biol. 1995; 131(5):1193-1203. (Biology). View Reference
  3. Loo LW, Berestecky JM, Kanemitsu MY, Lau AF. pp60src-mediated phosphorylation of connexin 43, a gap junction protein. J Biol Chem. 1995; 270(21):12751-12761. (Biology). View Reference
  4. Lucke T, Choudhry R, Thom R, Selmer IS, Burden AD, Hodgins MB. Upregulation of connexin 26 is a feature of keratinocyte differentiation in hyperproliferative epidermis, vaginal epithelium, and buccal epithelium. J Invest Dermatol. 1999; 112(3):354-361. (Biology: Immunohistochemistry). View Reference
  5. Toyofuku T, Akamatsu Y, Zhang H, Kuzuya T, Tada M, Hori M. c-Src regulates the interaction between connexin-43 and ZO-1 in cardiac myocytes. J Biol Chem. 2001; 276(3):1780-1788. (Biology: Immunoprecipitation, Western blot). View Reference
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610062 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.