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BD Pharmingen™ Biotin Mouse Anti-Human IP-10
Clone 6D4/D6/G2 (RUO)
Human IP-10 sandwich ELISA curve: The curve was generated by a sandwich ELISA using the Purified Mouse Anti-Human IP-10 4D5/A7/C5 (Cat. No. 555046) as capture antibody, doubling dilutions of Recombinant Human IP-10 (Cat. No. 551130) as standard, and Biotin Mouse Anti-Human IP-10 6D4/D6/G2 (Cat. No. 555048) as detection antibody. Streptavidin-HRP (Cat. No. 554066) plus TMB substrate (Cat. No. 555214) were used to develop the ELISA.
Human IP-10 sandwich ELISA curve: The curve was generated by a sandwich ELISA using the Purified Mouse Anti-Human IP-10 4D5/A7/C5 (Cat. No. 555046) as capture antibody, doubling dilutions of Recombinant Human IP-10 (Cat. No. 551130) as standard, and Biotin Mouse Anti-Human IP-10 6D4/D6/G2 (Cat. No. 555048) as detection antibody. Streptavidin-HRP (Cat. No. 554066) plus TMB substrate (Cat. No. 555214) were used to develop the ELISA.
Product Details
BD Pharmingen™
Human (QC Testing)
Mouse IgG2a, κ
Recombinant human IP-10 protein
ELISA Detection (Routinely Tested)
0.5 mg/ml
AB_395669
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures
ELISA: Biotin Mouse Anti-Human IP-10 (Cat. No. 555048) should be titrated to determine its optimal concentration for ELISA detection (0.5 - 2.0 µg/ml). To obtain linear standard curves, doubling dilutions of Recombinant Human IP-10 (Cat. No. 551130) ranging from ~2,500 to 39 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our web site, www.bdbiosciences.com/us/s/resources, and go to the protocols section under ELISA and ELISPOT.
Note 1: This ELISA pair shows no cross-reactivity with any of the cytokines or chemokines tested (human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-13, IL-15, IL-16, eotaxin, G-CSF, GM-CSF, GROα, GROβ, GROγ, IFN-γ, lymphotactin, MIG, MCP-1, MCP-2, MCP-3, MCP-4, MIP-1α, MIP-1β, NAP-2, PF-4, RANTES, TGF-β, TNF, LT-α and mouse Crg-2).
Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring human IP-10 in serum or plasma our Human IP-10 BD OptEIA™ ELISA Set (Cat. No. 550926) is specially formulated and recommended.
Immunofluorescent Staining and Flow Cytometric Analysis: The 6D4/D6/G2 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify and enumerate human IP-10 producing cells within mixed cell populations. The PE-conjugated 6D4/D6/G2 antibody (Cat. No. 555049) is suitable for these experiments. For specific methodology, please visit our web site, www.bdbiosciences.com/us/s/resources, and go to the protocols section under Cell Biology (WB, IP, IHC, IF) or Cytokines (Intracellular Staining).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
555048 Rev. 3
Antibody Details
6D4/D6/G2
The monoclonal antibody 6D4/D6/G2 reacts with human CXC chemokine, interferon gamma inducible protein 10 (IP-10). IP-10 is inducible in monocytes, keratinocytes and endothelial cells by IFN-γ. The immunogen used to generate the monoclonal antibody 6D4/D6/G2 was recombinant human IP-10 protein.
555048 Rev. 3
Format Details
Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
555048 Rev.3
Citations & References
View product citations for antibody "555048" on CiteAb
Development References (2)
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Luster AD, Ravetch JV. Biochemical characterization of a gamma interferon-inducible cytokine (IP-10). J Exp Med. 1987; 166(4):1084-1097. (Clone-specific). View Reference
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Luster AD, Unkeless JC, Ravetch JV. Gamma-interferon transcriptionally regulates an early-response gene containing homology to platelet proteins. Nature. 1985; 315(6021):672-676. (Clone-specific). View Reference
555048 Rev. 3
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.