Preparation And Storage
Recommended Assay Procedures
ELISA Capture:Biotin Mouse Anti-Human CD154 antibody (Cat. No. 552560) is useful as a detection antibody for a sandwich ELISA for measuring human CD154 (CD40L) protein levels.Biotin Mouse Anti-Human CD154 antibody should be titrated between 0.5 - 2 µg/ml to determine its optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of recombinant human soluble CD154 (CD40L), ranging from 2000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology see the protocols under "ELISA and ELISPOT" at our website: http://www.bdbiosciences.com/en-us/support/bd-biosciences-resource-library.
Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human cytokines: CD40, IL-1Ra, IL-1R1, IL-1RII, IL-1α, IL-1β, IL-2, IL-3, IL-4, sIL-4R, IL-5, IL-6, IL-6R, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, TNF-α, TNF-β, sTNFRI, sTNFRII, IFNγ, TRAIL, GM-CSF, TGFβ, Trx
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
hCD40L-M91 reacts with CD154 (CD40 ligand, CD40L), a 39 kDa type II membrane glycoprotein expressed on activated T cells. CD40CD40L interaction plays a very important role in T cell-dependent B-cell proliferation, differentiation, and memory cell formation. In addition, blocking of CD40-CD40L interaction has been demonstrated with soluble CD40, resulting in the inhibition of immunoglobulin isotype switching. It has been reported that patients with X-linked hyper-IgM syndrome have defective expression of functional CD40L due to a defective gene that encodes CD40L.
Development References (4)
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Fuleihan R, Ramesh N, Horner A, et al. Cyclosporin A inhibits CD40 ligand expression in T lymphocytes. J Clin Invest. 1994; 93(3):1315-1320. (Biology). View Reference
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Gray D, Dullforce P, Jainandunsing S. Memory B cell development but not germinal center formation is impaired by in vivo blockade of CD40-CD40 ligand interaction. J Exp Med. 1994; 180(1):141-155. (Biology). View Reference
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Nishioka Y, Lipsky PE. The role of CD40-CD40 ligand interaction in human T cell-B cell collaboration. J Immunol. 1994; 153(3):1027-1036. (Biology). View Reference
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van Kooten C, Gaillard C, Galizzi JP, et al. B cells regulate expression of CD40 ligand on activated T cells by lowering the mRNA level and through the release of soluble CD40. Eur J Immunol. 1994; 24(4):787-792. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
