Description
The Human Inflammatory Cytokine Standard contains a mixture of individually lyophilized recombinant proteins made up of recombinant human Interleukin (IL)-8, IL-1β, IL-6, IL-10, Tumor Necrosis Factor (TNF), and IL-12p70. The reconstituted Human Inflammatory Cytokine Standard is designed for use as a standard control with the BD™ Cytometric Bead Array (CBA) Human Inflammatory Cytokine Kit (Cat. No. 551811). Please refer to the manual included with the Human Inflammatory Cytokine CBA Kit for further information.
Preparation And Storage
Lyospheres from 1 vial of the Human Inflammatory Cytokine Standard, when reconstituted in 2.0 mL Assay Diluent, approximates the CBA activity of human Interleukin (IL)-8, IL-1β, IL-6, IL-10, Tumor Necrosis Factor (TNF), and IL-12p70 at a concentration of 5000 pg/mL. Following reconstitution, maintain the Human Inflammatory Cytokine Standard at 4°C and use within 12 hours. Any unused reconstituted standard must be discarded after this period (do not store or reuse).
Recommended Assay Procedures
The Human Inflammatory Cytokine Standard has been tested with the BD™ CBA Human Inflammatory Cytokine Kit (Cat. No. 551811) to assure protein amounts and function in the BD™ CBA assay. Investigators are encouraged to refer to the CBA kit manual or to the abbreviated instruction below (Preparation of Human Inflammatory Cytokine Standard Protocol). The Human Inflammatory Cytokine Standard should be reconstituted using Assay Diluent supplied with the aforementioned CBA kits (component no. 51-2432KC) or it may be purchased separately (Cat. No. 560104).
Preparation of Human Inflammatory Cytokine Standard : The Human Inflammatory Cytokine Standard is lyophilized and should be reconstituted and serially diluted before mixing with CBA capture beads and PE detection reagents.
1. Open one vial of the lyophilized Human Inflammatory Cytokine Standard. Transfer all the lyospheres to a polypropylene tube (BD Falcon™, Cat. No. 352097). Label the tube "Top Standard".
2. Reconstitute the standard with 2.0 mL of Assay Diluent. Allow the reconstituted standard to equilibrate for at least 15 minutes before making dilutions. Mix the reconstituted protein by pipette only. Do not vortex or mix vigorously.
3. Label additional 12 x 75 mm tubes (BD Falcon™, Cat. No. 352008) and arrange them in the following order: Top Standard, 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:128, and 1:256.
4. Add 300 µl of Assay Diluent to each of the dilution tubes.
5. Perform a serial dilution by transferring 300 µl from the Top Standard to the 1:2 dilution tube and mix thoroughly. Do not vortex, mix by pipet only. Continue making serial dilutions by transferring 300 µl from the 1:2 tube to the 1:4 tube and so on to the 1:256 tube and mix thoroughly. Prepare one tube containing only Assay Diluent to serve as the 0 pg/mL negative control.
Product Notices
- ProClin is a trademark of Rohm and Haas Company.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Warning: CBA lyophilized standard contains 0.02% (w/w) and Detection Reagent (Part A) contains 0.002% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/eye/face protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents/container in accordance with local/regional/national/international regulations.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
