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PE Rat anti-Mouse CD267 (TACI)
PE Rat anti-Mouse CD267 (TACI)

Flow cytometric analysis of PE rat anti-mouse CD267 on mouse spelenocytes.   Unstimulated mouse splenocytes were pre-incubated with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141).  The cells were then stained with FITC anti-mouse CD19 (Cat. No. 553785) and PE anti-mouse CD267 antibody (Clone 8F10, Cat. No. 558410; right panel) or PE-conjugated rat IgG2a (Clone R35-95, Cat. No. 553930; left panel). Flow cytometry was performed and the dot plots were derived from the gated events based on light scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD™ FACSCalibur flow cytometry instrument.

Flow cytometric analysis of PE rat anti-mouse CD267 on mouse spelenocytes.   Unstimulated mouse splenocytes were pre-incubated with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141).  The cells were then stained with FITC anti-mouse CD19 (Cat. No. 553785) and PE anti-mouse CD267 antibody (Clone 8F10, Cat. No. 558410; right panel) or PE-conjugated rat IgG2a (Clone R35-95, Cat. No. 553930; left panel). Flow cytometry was performed and the dot plots were derived from the gated events based on light scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD™ FACSCalibur flow cytometry instrument.

Product Details
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BD Pharmingen™
TACI; Taci;Tnfrsf13b; transmembrane activator and CAML interactor
Rat WF, also known as Wistar Furth IgG2a, κ
Mouse TACI Extracellular Domain Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
57916
AB_647234
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Recommended Assay Procedures

For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that the cells be pre-incubated with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. no.553141).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
558410 Rev. 1
Antibody Details
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8F10

The 8F10 monoclonal antibody specifically recognizes CD267 which is also known as transmembrane activator and calcium-modulating and cyclophilin ligand interactor(transmembrane activator and CAML interactor or TACI). CD267 is encoded by Tnfrsf13b (tumor necrosis factor receptor superfamily member 13B). CD267 is a type III transmembrane protein receptor that binds to the ligands BAFF (BLyS), and APRIL. BAFF overproduction is associated with lupus erythematosus and Sjögren's disease, two systemic autoimmune diseases. CD267 is expressed most notably on maturing subsets of splenic B cells such as transition type 2 (T2) and marginal zone (MZ) B cells and also in activated T-cells. In CD267 deficient mice, B cell numbers are increased and mice develop autoimmune disorders suggesting that CD267 plays a role in B cell regulation.

558410 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
558410 Rev.1
Citations & References
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Development References (4)

  1. Ng LG, Sutherland AP, Newton R, et al. B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells.. J Immunol. 2004; 173(2):807-17. (Biology). View Reference
  2. Siegel RM, Lenardo MJ.. To B or not to B: TNF family signaling in lymphocytes. Nat Immunol. 2001; 2(7):577-578. (Biology). View Reference
  3. Yan M, Wang H, Chan B, et al. Activation and accumulation of B cells in TACI-deficient mice.. Nat Immunol. 2001; 2(7):638-43. (Biology). View Reference
  4. von Bülow GU, Bram RJ. NF-AT activation induced by a CAML-interacting member of the tumor necrosis factor receptor superfamily. Science. 1997; 278(5335):138-141. (Biology). View Reference
View All (4) View Less
558410 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.