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PE Mouse Anti-Human HLA-DM
PE Mouse Anti-Human HLA-DM

Profile of intracellular staining of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA).

PE Mouse Anti-Human HLA-DM

Profile of intracellular staining of peripheral blood monocytes analyzed on a FACScan (BDIS, San Jose, CA).

Profile of intracellular staining of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA).

Profile of intracellular staining of peripheral blood monocytes analyzed on a FACScan (BDIS, San Jose, CA).

Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Intracellular staining (flow cytometry) (Routinely Tested)
20 µl
AB_396272
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

For multi-color staining for intracellular protein and cell surface antigens, please refer to Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols. The Cytofix/Cytoperm™ Kit (Catalog No. 554714) is recommended for intracellular staining of HLA-DM.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
555983 Rev. 5
Antibody Details
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MaP.DM1

Reacts with human leukocyte antigen-DM (HLA-DM), a non-classical MHC class II molecule expressed in the cytoplasm of antigen presenting cells (APC). HLA-DM is composed of alpha and beta subunits which form a similar structure as the classical class II molecules. HLA-DM catalyzes the dissociation of CLIP from MHC class II-CLIP complexes in vitro and facilitates the binding of antigenic peptides. It also prevents self-antigens from becoming stably complexed with class II molecules and being presented to T cells.

555983 Rev. 5
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
555983 Rev.5
Citations & References
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Development References (5)

  1. Denzin LK, Cresswell P. HLA-DM induces CLIP dissociation from MHC class II alpha beta dimers and facilitates peptide loading. Cell. 1995; 82(1):155-165. (Biology). View Reference
  2. Kropshofer H, Hämmerling GJ, Vogt AB. How HLA-DM edits the MHC class II peptide repertoire: survival of the fittest. Immunol Today. 1997; 18(2):77-82. (Biology). View Reference
  3. Kropshofer H, Vogt AB, Moldenhauer G, Hammer J, Blum JS, Hämmerling GJ. Editing of the HLA-DR-peptide repertoire by HLA-DM. EMBO J. 1996 November; 15(22):6144-6154. (Biology). View Reference
  4. Schafer PH, Green JM, Malapati S, Gu L, Pierce SK. HLA-DM is present in one-fifth the amount of HLA-DR in the class II peptide-loading compartment where it associates with leupeptin-induced peptide (LIP)-HLA-DR complexes. J Immunol. 1996 December; 157(12):5487-5495. (Biology). View Reference
  5. Wubbolts R, Fernandez-Bona M, Neefjes J. MHC class II molecules: transport pathways for antigen presentation. Trends Cell Biol. 1997 March; 7(3):115-118. (Biology). View Reference
View All (5) View Less
555983 Rev. 5

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.