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FITC Rat Anti-Mouse Vβ 14 T-Cell Receptor
FITC Rat Anti-Mouse Vβ 14 T-Cell Receptor

Two-color analysis of the expression of Vβ 14 TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotinylated 14-2 and PE-conjugated 145-2C11 (anti-CD3e, Cat. No. 553063/553064) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).

Two-color analysis of the expression of Vβ 14 TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotinylated 14-2 and PE-conjugated 145-2C11 (anti-CD3e, Cat. No. 553063/553064) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).

Product Details
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BD Pharmingen™
Mouse (QC Testing)
Rat F344, also known as Fischer, CDF IgM, κ
Mouse B10.A Helper T-Cell Clone J9.19
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_394738
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

For flow cytometry of cell suspensions from peripheral lymphoid tissue, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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Antibody Details
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14-2

The 14-2 antibody reacts with the Vβ 14 T-Cell Receptor (TCR) of mice having the a (e.g., C57BR, C57L, SJL, SWR), b (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2), and c (e.g., RIII) halpotypes of the Tcrb gene complex. Vβ 14 TCR-expressing T lymphocytes are completely eliminated in mice expressing I-E and the superantigens encoded by Mtv-2 endogenous provirus and/or MMTV-C3H, MMTV-GR, or MMTV-D2.GD exogenous virus. Recognition of these determinants by Vβ 14 TCR-expressing T cells is dependent upon presentation by I-E. Plate bound 14-2 antibody activates Vβ 14 TCR-bearing T cells.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

553258 Rev. 12
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
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Citations & References
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Development References (7)

  1. Acha-Orbea H, Shakhov AN, Scarpellino L, et al. Clonal deletion of V beta 14-bearing T cells in mice transgenic for mammary tumour virus. Nature. 1991; 350(6315):207-211. (Biology). View Reference
  2. Choi Y, Kappler JW, Marrack P. A superantigen encoded in the open reading frame of the 3' long terminal repeat of mouse mammary tumour virus. Nature. 1991; 350(6315):203-207. (Biology). View Reference
  3. Golovkina TV, Chervonsky A, Dudley JP, Ross SR. Transgenic mouse mammary tumor virus superantigen expression prevents viral infection. Cell. 1992; 69(4):637-645. (Clone-specific). View Reference
  4. Hodes RJ, Abe R. Mouse endogenous superantigens: Ms and Mls-like determinants encoded by mouse retroviruses.. Curr Protoc Immunol. 2001; Appendix 1:Appendix 1F. (Biology). View Reference
  5. Liao NS, Maltzman J, Raulet DH. Positive selection determines T cell receptor V beta 14 gene usage by CD8+ T cells. J Exp Med. 1989; 170(1):135-143. (Immunogen). View Reference
  6. Marrack P, Kushnir E, Kappler J. A maternally inherited superantigen encoded by a mammary tumour virus. Nature. 1991; 349(6309):524-526. (Biology). View Reference
  7. Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology). View Reference
View All (7) View Less
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.