The 25-9-17 monoclonal antibody specifically recognizes the β chain of the I-A[b] MHC class II alloantigen. It crossreacts with cells from mice of the H-2[d], H-2[p], and H-2[q] haplotypes. Reactivity with other haplotypes (eg, a, f, g7, k, r, s) has not been observed. The strain distribution of the antigen recognized by this reagent is similar or identical to that of anti-I-A[d] (Aβ[d]) mAb 34-5-3.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.