The MR11-1 monoclonal antibody specifically recognizes the Vβ 12 T-cell Receptor (TCR) of mice having the b haplotype (e.g., C57BL, C58, DBA/1) of the Tcrb gene complex1 The Tcrb-V12 gene locus is deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype. Vβ 12 TCR-bearing T lymphocytes are clonally eliminated in mice expressing I-E and superantigens encoded by Mtv-8 (Mlsf, Dvb11.1), Mtv-9 (Etc-1, Mlsf, Dvb11.2) and/or Mtv-11 (Mlsf, Dvb11.3) proviruses (eg, A, AKR, BALB/c, CBA/J, C3H, DBA/2). Activation of Vβ 12 TCR-expressing T cells by these determinants is dependent upon presentation by I-E. C57BL/6 spleen T cells expressing Vβ 12 TCR are among the predominant responders to MAIDS virus superantigen.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.