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Product Details
BD Pharmingen™
Mouse BALB/c IgG1, κ
ELISA, Flow cytometry, Isotype control (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen, Immunohistochemistry-paraffin, Immunohistochemistry-zinc-fixed (Tested During Development)
250 µg/ml
AB_10052281
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures
This antibody has been tested by ELISA and Flow cytometry to assure specificity and reactivity.
For immunohistochemical staining of acetone-fixed frozen sections or formalin-fixed paraffin sections, a dilution range of 1:12.5 - 1:50 is suggested.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Streptavidin HRP RUO
Size
50 ML
Cat No.
550946
Antibody Diluent for IHC RUO
Size
125 ML
Cat No.
559148
DAB Substrate Kit RUO
Size
500 Tests
Cat No.
550880
550878 Rev. 2
Antibody Details
MOPC-31C
The MOPC-31C antibody has unknown specificity. The transplantable plasmacytoma MOPC-31C was induced by intraperitoneal injection of mineral oils into BALB/c mice. It was adapted to continuous cell culture by alternate passage in animals.
550878 Rev. 2
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550878 Rev.2
Citations & References
View product citations for antibody "550878" on CiteAb
Development References (2)
-
Hay R, Caputo J, Chen TR, Macy M, McClintock P, Reid Y, ed. ATCC. Cell Lines and Hybridomas, Eighth Edition. 1994:75.
-
Sibinovic KH, Potter M, Hoostelaere, Rode B, Wax J, ed. Catalogue of plasmacytomas and other tumors of the lymphoreticular system, 3rd edition. Kensington, Maryland: Litton Bionetics, Inc; 1976:1-33.
550878 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.