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Purified Mouse Anti-ICBP90
Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG2a
Human ICBP90 aa. 199-298
Western blot (Routinely Tested), Fluorescence microscopy (Not Recommended)
97 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612264 Rev. 1
Antibody Details
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DNA topoisomerases are ubiquitous nuclear enzymes that alter the topology of DNA. Topoisomerase I produces transient single-stranded breaks in DNA, while topoisomerase II produces transient double-stranded breaks in DNA. These DNA breaks are important for DNA replication and chromosome condensation and segregation. Two types of topoisomerase II have been identified, TopoIIα and TopoIIβ. During interphase, TopoIIα is found in the nucleoplasm and nucleoli, and in mitosis, TopoIIα is found associated with metaphase chromosomes and centromeres. Transcriptional control of TopoIIα expression is regulated through multiple gene promoters, which include five inverted CCAAT boxes, one ATF binding sequence, and two GC-rich boxes. ICBP90 was identified in one-hybrid screen of proteins that can bind an inverted CCAAT box. ICBP90 is expressed highest in proliferative tissues such as thymus, liver, and bone marrow. Overexpression of ICBP90 in COS-1-transfected cells leads to enhanced expression of TopoIIα. ICBP90 co-localizes with TopoIIα in proliferating areas of appendix and in high grade breast carcinomas. Thus, ICBP90 may be an important transcripitonal regulator of TopoIIα expression in proliferating cells.

612264 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
612264 Rev.1
Citations & References
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View product citations for antibody "612264" on CiteAb

Development References (2)

  1. Hopfner R, Mousli M, Garnier JM, et al. Genomic structure and chromosomal mapping of the gene coding for ICBP90, a protein involved in the regulation of the topoisomerase IIalpha gene expression. Gene. 2001; 266(1-2):15-23. (Biology). View Reference
  2. Hopfner R, Mousli M, Jeltsch JM, et al. ICBP90, a novel human CCAAT binding protein, involved in the regulation of topoisomerase IIalpha expression. Cancer Res. 2000; 60(1):121-128. (Biology). View Reference
612264 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.