The S-HCL-3 monoclonal antibody specifically binds to CD11c which is encoded by ITGAX (Integrin alpha X, complement component 3 receptor 4 subunit). CD11c is a ~150 kDa type I transmembrane glycoprotein that is expressed on dendritic cells, monocytes, macrophages, granulocytes, NK cells and subsets of B and T cells. CD11c is also present on hairy-cell leukemias and on some acute myeloid leukemias (AML). The S-HCL-3 antibody stains sinus histiocytes of lymph node, tonsil, and spleen; macrophages in the cortex and medulla of thymus; Kupffer cells in liver; elongated intertubular cells in kidney; and alveolar macrophages in lung tissue. In malignant tissue, the antibody stains true histiocytic lymphomas; it does not stain carcinomas or T or B lymphomas. CD11c associates with CD18 (ITGB2/Integrin beta-2) to form the CD11c/CD18 heterodimer which is likewise known as Leukocyte adhesion receptor p150,95 or Complement receptor type 4 (CR4). Ligands for this heterodimeric adhesion receptor include fibrinogen, iC3b, CD23, and CD54 (ICAM-1).
The antibody was conjugated to an oligonucleotide that contains an antibody clone-specific barcode (ABC) flanked by a poly-A tail on the 3' end and a PCR handle (PCR primer binding site) on the 5' end. The ABC for this antibody was designed to be used with other BD AbSeq oligonucleotides conjugated to other antibodies. All AbSeq ABC sequences were selected in silico to be unique from human and mouse genomes, have low predicted secondary structure, and have high Hamming distance within the BD AbSeq portfolio, to allow for sequencing error correction and unique mapping. The poly-A tail of the oligonucleotide allows the ABC to be captured by the BD Rhapsody™ system. The 5' PCR handle allows for efficient sequencing library generation for Illumina sequencing platforms.
NOTE: The BD Rhapsody Single-Cell Analysis System must be used with the BD Rhapsody Express Instrument.