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Purified Mouse Anti-Human c-Myc with Control

BD Pharmingen™ Purified Mouse Anti-Human c-Myc with Control

Purified Mouse Anti-Human c-Myc with Control
Western blot analysis of c-myc. Lysate from Jurkat cells was probed with anti-c-myc (clone 9E10, Comp. No. 51-1485GR) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). C-myc is identified as a band of 62 kDa.
Western blot analysis of c-myc. Lysate from Jurkat cells was probed with anti-c-myc (clone 9E10, Comp. No. 51-1485GR) at concentrations of 2.0 (lane 1), 1.0 (lane 2), and 0.5 µg/ml (lane 3). C-myc is identified as a band of 62 kDa.
Product Details
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BD Pharmingen™
Human (QC Testing)
Western blot (Routinely Tested), Electron microscopy, Fluorescence microscopy, Immunohistochemistry-paraffin (Reported), Immunohistochemistry-frozen (Not Recommended)

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

It is particularly useful for western blot analysis of myc-tagged recombinant proteins, and recombinant c-myc. Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (Comp. No. 51-16526N; store lysate at -20 °C). Additional control lysate is sold separately.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
551101 Rev. 3
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Description Quantity/Size Part Number EntrezGene ID
Purified Mouse Anti-Human c-Myc 50 µg (1 ea) 51-1485GR N/A
Jurkat Cell Lysate 50 µg (1 ea) 51-16526N N/A
551101 Rev. 3
Citations & References
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Development References (13)

  1. Blackwood EM, Eisenman RN. Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc. Science. 1991; 251(4998):1211-1217. (Biology). View Reference
  2. Campbell AM, Kessler PD, Fambrough DM. The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca(2+)-ATPases are on opposite sides of the membrane. J Biol Chem. 1992; 267(13):9321-9325. (Clone-specific: Fluorescence microscopy, Western blot). View Reference
  3. DePinho RA, Schreiber-Agus N, Alt FW. myc family oncogenes in the development of normal and neoplastic cells. Adv Cancer Res. 1991; 57:1-46. (Biology). View Reference
  4. Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol. 1985; 5(12):3610-3616. (Immunogen: Western blot). View Reference
  5. Gosney JR, Field JK, Gosney MA, Lye MD, Spandidos DA, Butt SA. c-myc oncoprotein in bronchial carcinoma: expression in all major morphological types. Anticancer Res. 1990; 10(3):623-628. (Clone-specific: Electron microscopy, Immunohistochemistry). View Reference
  6. Karayiannis M, Yiagnisis M, Papadimitriou K, Field JK, Spandidos DA. Evaluation of the ras and myc oncoproteins in benign gastric lesions. Anticancer Res. 1990; 10(5A):1127-1134. (Clone-specific: Immunohistochemistry). View Reference
  7. Liu ZG, Hsu H, Goeddel DV, Karin M. Dissection of TNF receptor 1 effector functions: JNK activation is not linked to apoptosis while NF-kappaB activation prevents cell death. Cell. 1996; 87(3):565-576. (Clone-specific: Western blot). View Reference
  8. Lukas J, Parry D, Aagaard L, et al. Retinoblastoma-protein-dependent cell-cycle inhibition by the tumour suppressor p16. Nature. 1995; 375(6531):503-506. (Clone-specific: Western blot). View Reference
  9. Royds JA, Sharrard RM, Wagner B, Polacarz SV. Cellular localisation of c-myc product in human colorectal epithelial neoplasia. J Pathol. 1992; 166(3):225-233. (Clone-specific: Immunohistochemistry). View Reference
  10. Spandidos DA, Yiagnisis M, Papadimitriou K, Field JK. ras, c-myc and c-erbB-2 oncoproteins in human breast cancer. Anticancer Res. 1989; 9(5):1385-1394. (Clone-specific: Immunohistochemistry). View Reference
  11. Tiniakos D, Spandidos DA, Kakkanas A, Pintzas A, Pollice L, Tiniakos G. Expression of ras and myc oncogenes in human hepatocellular carcinoma and non-neoplastic liver tissues. Anticancer Res. 1989; 9(3):715-722. (Clone-specific: Immunohistochemistry). View Reference
  12. Tosca A, Linardopoulos S, Malliri A, Hatziolou E, Nicolaidou A, Spandidos DA. Implication of the ras and myc oncoproteins in the pathogenesis of mycosis fungoides. Anticancer Res. 1991; 11(4):1433-1438. (Clone-specific: Immunohistochemistry). View Reference
  13. Williams AR, Piris J, Wyllie AH. Immunohistochemical demonstration of altered intracellular localization of the C-Myc oncogene product in human colorectal neoplasms. J Pathol. 1990; 160(4):287-293. (Clone-specific: Immunohistochemistry). View Reference
View All (13) View Less
551101 Rev. 3

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.