BD Transduction Laboratories™ Purified Mouse Anti-Glutamine Synthetase
Clone 6/Glutamine Synthetase (RUO)
Product Details
BD Transduction Laboratories™
Glutamate-Ammonia Ligase; GLUL; GLNS
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG2a
Human Glutamine Synthetase aa. 1-373
Western blot (Routinely Tested), Bioimaging, Immunohistochemistry (Tested During Development), Immunofluorescence, Immunoprecipitation (Not Recommended)
45 kDa
250 µg/ml
AB_397879
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Recommended Assay Procedures
For Western blot and IHC: Please refer to http://www.bdbiosciences.com/resources/cellbiology/index.jsp
For Bioimaging: Please refer to http://www.bdbiosciences.com/resources/cellularimaging/index.jsp
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Triton is a trademark of the Dow Chemical Company.
610517 Rev. 2
Antibody Details
6/Glutamine Synthetase
Glutamine synthetase catalyzes the amination of glutamic acid to form glutamine. It is found in mammals as an octamer of identical 45 kDa subunits. Glutamine synthetase activity is a useful marker for astrocytes and an important differentiation feature in retina. It is also considered to be a key enzyme in the recycling of the neurotransmitter glutamate.
610517 Rev. 2
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610517 Rev.2
Citations & References
View product citations for antibody "610517" on CiteAb
Development References (5)
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Fei ZL, D'Ambrosio C, Li S, Surmacz E, Baserga R. Association of insulin receptor substrate 1 with simian virus 40 large T antigen. Mol Cell Biol. 1995; 15(8):4232-4239. (Biology: Immunoprecipitation). View Reference
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Kentroti S, Baker R, Lee K, Bruce C, Vernadakis A. Platelet-activating factor increases glutamine synthetase activity in early and late passage C-6 glioma cells. J Neurosci Res. 1991; 28(4):497-506. (Biology). View Reference
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Kronfeld I, Kazimirsky G, Lorenzo PS, Garfield SH, Blumberg PM, Brodie C. Phosphorylation of protein kinase Cdelta on distinct tyrosine residues regulates specific cellular functions. J Biol Chem. 2000; 275(45):35491-35498. (Biology: Western blot). View Reference
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Labow BI, Souba WW, Abcouwer SF. Glutamine synthetase expression in muscle is regulated by transcriptional and posttranscriptional mechanisms. Am J Physiol. 1999; 276(6):E1136-E1145. (Biology: Western blot). View Reference
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Vardimon L, Fox LE, Cohen-Kupiec R, Degenstein L, Moscona AA. Expression of v-src in embryonic neural retina alters cell adhesion, inhibits histogenesis, and prevents induction of glutamine synthetase. Mol Cell Biol. 1991; 11(10):5275-5284. (Biology). View Reference
610517 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.