Description
Mouse erythropoietin (EPO) is a 30 kD heavily glycosylated protein containing 166 amino acids. The carbohydrate residues compose approximately 30% of the molecule by weight.3 It shares 80% and 95% homology with human and rat EPO, respectively. EPO functions as the survival and proliferation factors of late erythroid progenitor cells (CFU-E). In adult mammals, EPO is synthesized almost exclusively in the kidneys.
Formulation and Purity
Recombinant mouse EPO is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, and containing 1 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The recombinant mouse EPO is > 95% pure, as determined by SDS-PAGE and an absorbance assay based on Beers-Lambert law. The endotoxin level is ≤ 0.1 ng per µg of mouse EPO, as measured in a chromogenic LAL assay.
Preparation And Storage
Recommended Assay Procedures
Biological Activity
Measured using TF-1 indicator cells
Specific Activity: 0.1 - 1.0 × 10^8 Units/mg (Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation).
ED50: 0.1 - 1.0 ng/ml; Observed linear dose response range: >100 fold
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Development References (7)
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Jacobson LO, Goldwasser E, Fried W, Plzak L. Role of the kidney in erythropoiesis. Nature. 1957; 179(4560):633-634. (Biology). View Reference
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Kitamura T, Tange T, Terasawa T, et al. Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J Cell Physiol. 1989; 140(2):323-334. (Biology). View Reference
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Krantz SB. Erythropoietin. Blood. 1991; 77(3):419-434. (Biology). View Reference
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McDonald JD, Lin FK, Goldwasser E. Cloning, sequencing, and evolutionary analysis of the mouse erythropoietin gene. Mol Cell Biol. 1986; 6(3):842-848. (Biology). View Reference
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Nagao, M., H. Suga, et al. Nucleotide sequence of rat erythropoietin. Biochim Biophys Acta. 1992; 1171(1):99-102. (Biology). View Reference
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Shoemaker CB, Mitsock LD. Murine erythropoietin gene: cloning, expression, and human gene homology. Mol Cell Biol. 1986; 6(3):849-858. (Biology). View Reference
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Wu H, Liu X, Jaenisch R, Lodish HF. Generation of committed erythroid BFU-E and CFU-E progenitors does not require erythropoietin or the erythropoietin receptor. Cell. 1995; 83(1):59-67. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
