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Biotin Rat Anti-Mouse IgG2b
Product Details
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BD Pharmingen™
Igh-3; gamma2b; Immunoglobulin heavy constant gamma 2B
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Pooled Mouse Ig
ELISA Detection (Routinely Tested), Flow cytometry, Immunohistochemistry-frozen (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

ELISA: For the mouse IgG2b sandwich ELISA, Biotin Rat Anti-Mouse IgG2b is optimal for detection (~2 µg/ml) using Purified Rat Anti-Mouse IgG2b, (Cat. No. 553396) for capture, with Purified Mouse IgG2b, κ Isotype Control, (Cat. No. 557351) as the standard.

Immunofluorescent staining: Biotin Rat Anti-Mouse IgG2b may be used as a primary or secondary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic IgG2b, we recommend FITC Rat Anti-Mouse IgG2b, (Cat. No. 553395).

Immunohistochemistry: For IHC, we recommend the use of Biotin Rat Anti-Mouse IgG2b, (Cat. No. 550333) in our special formulation for immunohistochemistry.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  5. Please refer to for technical protocols.
553393 Rev. 13
Antibody Details
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The R12-3 antibody recognizes an epitope in  the CH3 domain of mouse IgG2b of Igh-C[a] and Igh-C[b] haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with R12-3 mAb.

553393 Rev. 13
Format Details
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Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
553393 Rev.13
Citations & References
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Development References (1)

  1. Wu K, Chen A, Tan P, Pan ZQ. The Nedd8-conjugated ROC1-CUL1 core ubiquitin ligase utilizes Nedd8 charged surface residues for efficient polyubiquitin chain assembly catalyzed by Cdc34.. J Biol Chem. 2002; 277(1):516-27. (Biology). View Reference
553393 Rev. 13

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.