Rat TNF Flex Set

BD™ Cytometric Bead Array (CBA) Rat TNF Flex Set

Name: Rat TNF Flex Set
Brand: BD™ Cytometric Bead Array (CBA)
SKU: 558309

(RUO)

Protocol Library

Scientific Resources

Figure 1. Example BD CBA Rat TNF Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software.

Reproducibility: The inter-assay and intra-assay reproducibility were determined for the BD CBA Rat TNF Flex Set by evaluating ten replicates of three different sample levels (inter-assay) and two replicates of three different sample levels from four separate experiments (intra-assay) respectively.

Recovery: Cell culture supernatant and pooled rat serum were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum samples were diluted 1:4 before the protein was spiked into each. Serum used was commercially available pooled rat serum.

Linearity: Cell culture supernatant and 1:4 diluted pooled rat serum were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.

Figure 1. Example BD CBA Rat TNF Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using FCAP Array Software.

Protocol Library

Scientific Resources

Product Details

Brand: BD™ Cytometric Bead Array (CBA)

Bead Position: C8

Assay Range: 40-10,000 pg/mL

Reactivity: Rat (QC Testing), Mouse (Tested in Development)

Regulatory Status: RUO

RRID: AB_2869152

Description

The BD™ CBA Rat TNF Flex Set is a bead-based immunoassay capable of measuring rat tumor necrosis factor (TNF) in serum and cell culture supernatant samples. Rat and mouse reactivity was determined by testing samples with the BD CBA Rat TNF Flex Set. For quantitation of mouse TNF, we recommend the BD™ CBA Mouse TNF Flex Set (Cat. No. 558299). For more information on bead-based immunoassays, refer to the product insert for the BD CBA Mouse/Rat Soluble Protein Master Buffer Kit (Cat. No. 558266 or 558267).

Preparation And Storage

This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 mL polypropylene tube for reconstitution. When reconstituted in 4.0 mL Assay Diluent, the standard has a protein concentration of 10,000 pg/mL. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.

Recommended Assay Procedures

The BD CBA Rat TNF Flex Set must be used in conjunction with a BD CBA Mouse/Rat Soluble Protein Master Buffer Kit (Cat. No. 558266, 100 tests, or 558267, 500 tests), a flow cytometer, and FCAP Array™ Software. Detailed instructions on the use of this product can be found in the manual for the BD CBA Mouse/Rat Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Rat TNF Flex Set will be 10,000 pg/mL. An example standard curve is shown in figure 1.

The BD CBA Rat TNF Flex Set should not be used in the same assay well with any non-BD CBA Rat Soluble Protein Flex Set reagents (such as BD CBA Human or Mouse Soluble Protein or Cell Signaling Flex Sets). For an updated assay compatibility chart for the BD CBA Rat Soluble Protein Flex Sets, please refer to the BD CBA Flex Set System homepage at http://www.bdbiosciences.com/cbasetup.

Performance

Limit of Detection: The theoretical limit of detection is 27.7 pg/mL and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/mL, n=30) + 2 standard deviations.

Product Notices

ProClin is a trademark of Rohm and Haas Company.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Warning: CBA lyophilized standard contains 0.02% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/eye/face protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents/container in accordance with local/regional/national/international regulations.

558309 Rev. 9

Components

Description	Quantity/Size	Part Number	EntrezGene ID
Rat TNF PE Detection Reagent	N/A	51-9004160	N/A
Rat TNF Standard	1 Vial(s) (2 ea)	51-9004115	N/A
Rat TNF Capture Bead C8	1 Vial(s) (1 ea)	51-9005325	N/A

558309 Rev. 9

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.