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Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- CF™ is a trademark of Biotium, Inc.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
Companion Products
The AH9R7 monoclonal antibody specifically binds to the Interleukin-9 Receptor (IL-9R, IL-9Rα) also known as CD129. CD129 is a Type I transmembrane glycoprotein and a Type I Cytokine Receptor (Hemopoietin) Superfamily member. The high-affinity, signaling IL-9 Receptor complex is comprised of CD129 (IL-9 Receptor alpha chain subunit) and CD132 (common γ chain/γc). CD129 is expressed by hematopoietic progenitors, thymocytes, T cells, B cells, macrophages, eosinophils, mast cells, epithelia cells, muscle cells and neurons. IL-9 signals through the IL-9R complex (ie, through JAK, STAT and related signal pathways) to regulate the growth, proliferation, differentiation and survival of myeloid and erythroid progenitor cells, thymocytes, T cells, B cells and some tumor cells.
Development References (6)
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De Smedt M, Verhasselt B, Kerre T, et al. Signals from the IL-9 receptor are critical for the early stages of human intrathymic T cell development. J Immunol. 2000; 164(4):1761-1767. (Clone-specific: Inhibition). View Reference
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Demoulin JB, Uyttenhove C, Van Roost E, et al. A single tyrosine of the interleukin-9 (IL-9) receptor is required for STAT activation, antiapoptotic activity, and growth regulation by IL-9. Mol Cell Biol. 1996; 16(9):4710-4716. (Clone-specific: Flow cytometry, Immunoprecipitation). View Reference
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Grasso L, Huang M, Sullivan CD, et al. Molecular analysis of human interleukin-9 receptor transcripts in peripheral blood mononuclear cells. Identification of a splice variant encoding for a nonfunctional cell surface receptor. J Biol Chem. 1998; 273(37):24016-24024. (Clone-specific: Flow cytometry, IC/FCM Block, Immunohistochemistry, Immunoprecipitation). View Reference
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Pilette C, Ouadrhiri Y, Van Snick J, et al. IL-9 inhibits oxidative burst and TNF-alpha release in lipopolysaccharide-stimulated human monocytes through TGF-beta. J Immunol. 2002; 168(8):4103-4111. (Clone-specific: Flow cytometry, Inhibition). View Reference
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Pilette C, Ouadrhiri Y, Van Snick J, et al. Oxidative burst in lipopolysaccharide-activated human alveolar macrophages is inhibited by interleukin-9. Eur Respir J. 2002; 20:1198-1205. (Clone-specific: Functional assay, Inhibition). View Reference
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Wilhelm C, Turner JE, Van Snick J, Stockinger B. The many lives of IL-9: a question of survival?. Nat Immunol. 2012; 13(7):637-641. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.