Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The OX-39 monoclonal antibody specifically binds to the α chain of the IL-2 Receptor (IL-2Rα, IL2RA) on activated T cells, T regulatory cells, and thymic and splenic dendritic cells. CD25 has also been detected on rat intestinal epithelial cells. It has been reported that OX-39 mAb weakly blocks binding of IL-2 to T-cell blasts and that it blocks IL-2 stimulated epithelial cell migration in an in vitro model of wound healing.
Development References (5)
-
Dignass AU, Podolsky DK. Interleukin 2 modulates intestinal epithelial cell function in vitro. Exp Cell Res. 1996; 225(2):422-429. (Clone-specific: Blocking). View Reference
-
Hillebrands JL, Whalen B, Visser JT, et al. A regulatory CD4+ T cell subset in the BB rat model of autoimmune diabetes expresses neither CD25 nor Foxp3.. J Immunol. 2006; 177(11):7820-32. (Biology). View Reference
-
Josien R, Heslan M, Soulillou JP, Cuturi MC. Rat spleen dendritic cells express natural killer cell receptor protein 1 (NKR-P1) and have cytotoxic activity to select targets via a Ca2+-dependent mechanism. J Exp Med. 1997; 186(3):467-472. (Clone-specific). View Reference
-
Paterson DJ, Jefferies WA, Green JR. Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts. Mol Immunol. 1987; 24(12):1281-1290. (Immunogen: Blocking, Immunofluorescence, Immunohistochemistry). View Reference
-
Tellides G, Dallman MJ, Kupiec-Weglinski JW, Diamantstein T, Morris PJ. Functional blocking of the interleukin-2 receptor (IL-2R) may be important in the efficacy of IL-2R antibody therapy. Transplant Proc. 1987; 19(5):4231-4233. (Clone-specific: Blocking). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.