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PE Rat Anti-Mouse DCIR1 (Clec4a2)
PE Rat Anti-Mouse DCIR1 (Clec4a2)

Multicolor flow cytometric analysis of DCIR1 (Clec4a2) expression on mouse splenic leucocyte populations. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; dashed line histograms) or PE Rat Anti-Mouse DCIR1 (Clec4a2) antibody (Cat. No. 566810; solid line histograms) at 0.5 µg/test and either 1) BD Horizon™ BUV395 Hamster Anti-Mouse CD11c (Cat. No. 564080) and FITC Rat Anti-Mouse I-A/I-E (Cat. No. 553623) antibodies to permit rectangular gating on conventional CD11c+ and I-A/I-E+ dendritic cells (cDCs; Top Left Plot) or 2) BD Horizon BUV395 Rat Anti-CD11b (Cat. No. 563553) and FITC Rat Anti-Mouse Ly-6G (Cat. No. 551460) antibodies to permit gating on CD11b+ and Ly-6G+ Neutrophils (Bottom Left Plot) as shown.

       Histograms showing DCIR1 (Clec4a2) expression [or Ig Isotype control staining] for either gated cDCs (Top Right Plot) or Neutrophils (Bottom Right Plot) are displayed. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Multicolor flow cytometric analysis of DCIR1 (Clec4a2) expression on mouse splenic leucocyte populations. C57BL/6 mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; dashed line histograms) or PE Rat Anti-Mouse DCIR1 (Clec4a2) antibody (Cat. No. 566810; solid line histograms) at 0.5 µg/test and either 1) BD Horizon™ BUV395 Hamster Anti-Mouse CD11c (Cat. No. 564080) and FITC Rat Anti-Mouse I-A/I-E (Cat. No. 553623) antibodies to permit rectangular gating on conventional CD11c+ and I-A/I-E+ dendritic cells (cDCs; Top Left Plot) or 2) BD Horizon BUV395 Rat Anti-CD11b (Cat. No. 563553) and FITC Rat Anti-Mouse Ly-6G (Cat. No. 551460) antibodies to permit gating on CD11b+ and Ly-6G+ Neutrophils (Bottom Left Plot) as shown.

       Histograms showing DCIR1 (Clec4a2) expression [or Ig Isotype control staining] for either gated cDCs (Top Right Plot) or Neutrophils (Bottom Right Plot) are displayed. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Product Details
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BD Pharmingen™
Clec4a; Clec4a2; Clecsf6; DCIR; Dcir1
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2a, κ
Mouse DCIR1 Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2869880
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566810 Rev. 1
Antibody Details
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TKKT-1

The TKKT-1 monoclonal antibody specifically recognizes Dendritic cell immunoreceptor 1 (DCIR1), a single-pass type II membrane glycoprotein that is encoded by Clec4a2 (C-type lectin domain family 4, member a2). DCIR1 (Clec4a2) is a member of the dendritic cell immunoreceptor (DCIR) family which contains four dendritic cell inhibitory receptors (DCIR1-4) and two activating receptors (DCAR1, 2). The C-terminal extracellular region of DCIR1 (Clec4a2) has one C-type lectin carbohydrate recognition domain (CRD) that is followed by a transmembrane region. Its cytoplasmic tail contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that can transmit inhibitory signals. DCIR1 (Clec4a2) is expressed on myeloid cells including dendritic cells, macrophages, neutrophils and eosinophils. C-type lectins bind to carbohydrates in a calcium-dependent manner. This inhibitory receptor may be involved in the regulation of immune responses.

        

566810 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566810 Rev.1
Citations & References
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Development References (5)

  1. Bates EE, Fournier N, Garcia E, et al. APCs express DCIR, a novel C-type lectin surface receptor containing an immunoreceptor tyrosine-based inhibitory motif.. J Immunol. 1999; 163(4):1973-83. (Biology). View Reference
  2. Flornes LM, Bryceson YT, Spurkland A, Lorentzen JC, Dissen E, Fossum S. Identification of lectin-like receptors expressed by antigen presenting cells and neutrophils and their mapping to a novel gene complex.. Immunogenetics. 2004; 56(7):506-17. (Biology). View Reference
  3. Fujikado N, Saijo S, Yonezawa T, et al. Dcir deficiency causes development of autoimmune diseases in mice due to excess expansion of dendritic cells.. Nat Med. 2008; 14(2):176-80. (Biology). View Reference
  4. Kanazawa N, Tashiro K, Inaba K, Miyachi Y. Dendritic cell immunoactivating receptor, a novel C-type lectin immunoreceptor, acts as an activating receptor through association with Fc receptor gamma chain.. J Biol Chem. 2003; 278(35):32645-52. (Biology). View Reference
  5. Kishimoto A, Watanabe M, Terauchi K, et al. Ubiquitous versus restricted expression of the two mouse dendritic cell C-type lectin receptors, DCIR1 and DCAR2, among myeloid cells.. Biochem Biophys Res Commun. 2015; 467(2):383-8. (Immunogen: Cell separation). View Reference
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566810 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.