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Two-color flow cytometric analysis of PLZF expression by human peripheral blood lymphocytes and mouse thymocytes. Left Panel: Human peripheral blood mononuclear cells were fixed and permeabilized using BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). The cells were subsequently washed with BD Perm/Wash™ Buffer (Cat. No. 554723) and stained with Alexa Fluor® 647 Mouse Anti-Human NCAM-1 (CD56) antibody (Cat. No. 563443) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-PLZF antibody (Cat. No. 564850; Right Plot). The two-color flow cytometric contour plots showing the correlated expression of CD56 versus PLZF (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Right Panel: BALB/c mouse thymocytes were stained with APC Hamster Anti-Mouse CD3e antibody (Cat. No. 553066/561826). The cells were washed and similarly fixed and permeabilized. The cells were then washed with BD Perm/Wash™ Buffer and stained with either PE Mouse IgG1, κ Isotype Control (Left Plot) or PE Mouse Anti-PLZF antibody (Right Plot). The two-color flow cytometric contour plots showing the correlated expression of CD3e versus PLZF (or Ig Isotype control staining) were derived from gated events with the forward and side light- scatter characteristics of intact thymocytes. Flow cytometric analyses were performed using a BD LSRFortessa™ Cell Analyzer System.
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BD Pharmingen™ PE Mouse Anti-PLZF
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The R17-809 monoclonal antibody recognizes promyelocytic leukemia zinc finger protein (PLZF). PLZF is also known as zinc finger and BTB domain containing 16 (ZBTB16) and Zinc finger protein 145. PLZF is a member of the BTB/POZ-ZF family of transcription factors that includes Th-POK (Zbtb7b) which is involved in CD4+ T cell fate determination. PLZF serves multiple functions. In the immune system, PLZF is involved in the developmental regulation of innate immune lymphocytes including NKT cells, a subset of γδ T cells, and mucosal associated invariant T (MAIT) cells. The R17-809 hybridoma was generated from a mouse immunized with recombinant mouse PLZF protein. The R17-809 antibody crossreacts with both mouse and human PLZF.

Development References (3)
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Alonzo ES, Gottschalk RA, Das J, et al. Development of promyelocytic zinc finger and ThPOK-expressing innate gamma delta T cells is controlled by strength of TCR signaling and Id3. J Immunol. 2010; 184(3):1268-1279. (Biology). View Reference
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Alonzo ES, Sant'Angelo DB. Development of PLZF-expressing innate T cells. Curr Opin Immunol. 2011; 23(2):220-227. (Biology). View Reference
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Savage AK, Constantinides MG, Han J, et al. The transcription factor PLZF directs the effector program of the NKT cell lineage. Immunity. 2008; 29(3):391-403. (Biology). View Reference
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