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PE Mouse Anti-Human Vimentin
PE Mouse Anti-Human Vimentin

Flow cytometric analysis of vimentin in human embryonic stem (ES) cells differentiated to a neural fate. H9 human ES cells (WiCell, Madison, WI) passage 48 were differentiated with media containing Noggin (R&D Systems) for 17 days (Yuan SH, Martin J, Elia J, et al, 2011), fixed in BD Cytofix™ buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm buffer III (Cat. No. 558050), and stained with matching concentrations of either PE Mouse IgG1, κ isotype control (dashed line, Cat. No. 554680) or PE Mouse Anti-Vimentin monoclonal antibody (Cat. No. 562337, solid line). Histograms were derived from gated events based on light scattering characteristics of the H9-derived ectoderm. Flow cytometry was performed on a BD™ LSR II flow cytometry system.

PE Mouse Anti-Human Vimentin

Flow cytometric analysis of vimentin in mesenchymal stem cells (MSC). MSC (Lonza) were fixed in BD Cytofix™ buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm buffer III (Cat. No. 558050), and stained with matching concentrations of either PE Mouse IgG1, κ isotype control (Cat. No. 554680, dashed line) or PE Mouse Anti-Vimentin monoclonal antibody (Cat. No. 562337, solid line). Histograms were derived from gated events based on light scattering characteristics of MSC. Flow cytometry was performed on a BD™ LSR II flow cytometry system.

Flow cytometric analysis of vimentin in human embryonic stem (ES) cells differentiated to a neural fate. H9 human ES cells (WiCell, Madison, WI) passage 48 were differentiated with media containing Noggin (R&D Systems) for 17 days (Yuan SH, Martin J, Elia J, et al, 2011), fixed in BD Cytofix™ buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm buffer III (Cat. No. 558050), and stained with matching concentrations of either PE Mouse IgG1, κ isotype control (dashed line, Cat. No. 554680) or PE Mouse Anti-Vimentin monoclonal antibody (Cat. No. 562337, solid line). Histograms were derived from gated events based on light scattering characteristics of the H9-derived ectoderm. Flow cytometry was performed on a BD™ LSR II flow cytometry system.

Flow cytometric analysis of vimentin in mesenchymal stem cells (MSC). MSC (Lonza) were fixed in BD Cytofix™ buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm buffer III (Cat. No. 558050), and stained with matching concentrations of either PE Mouse IgG1, κ isotype control (Cat. No. 554680, dashed line) or PE Mouse Anti-Vimentin monoclonal antibody (Cat. No. 562337, solid line). Histograms were derived from gated events based on light scattering characteristics of MSC. Flow cytometry was performed on a BD™ LSR II flow cytometry system.

Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1
Purified Cow Lens Vimentin
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl
AB_10897167
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
562337 Rev. 1
Antibody Details
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RV202

Intermediate filaments (IF) are a subset of cytoskeletal proteins which function to give overall structural integrity to the plasma membrane as well to organize cells into specific tissues. IF proteins can be divided into six major types based upon the similarity in sequence. Vimentin belongs to the type III category of IF proteins which are expressed in leukocytes, blood vessel endothelial cells, some epithelial cells, and mesenchymal cells. Vimentin is also expressed together with several other IF proteins during the early stages of development. Vimentin is exchanged for the tissue-specific intermediate filament type as differentiation proceeds. Vimentin migrates in SDS/PAGE as a ~57 kDa protein.

562337 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
562337 Rev.1
Citations & References
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Development References (6)

  1. Broers JL, Carney DN, Klein Rot M, et al . Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies. J Cell Sci. 1986; 83:37-60. (Clone-specific: Immunofluorescence, Western blot). View Reference
  2. Lodish HF. Molecular cell biology, 4th ed.. New York: W.H. Freeman; 2000:795-847.
  3. Pieper FR, Schaart G, Krimpenfort PJ, et al. Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells. J Cell Biol. 1989; 108(3):1009-1024. (Clone-specific: Electron microscopy, Immunofluorescence, Immunohistochemistry, Western blot). View Reference
  4. Raats JM, Pieper FR, Vree Egberts WT, Verrijp KN, Ramaekers FC, Bloemendal H. Assembly of amino-terminally deleted desmin in vimentin-free cells. J Cell Biol. 1990; 111(5):1971-1985. (Clone-specific: Immunohistochemistry). View Reference
  5. Viebahn C, Lane EB, Ramaekers FC. Keratin and vimentin expression in early organogenesis of the rabbit embryo. Cell Tissue Res. 1988; 253(3):553-562. (Clone-specific: Immunofluorescence). View Reference
  6. Yuan SH, Martin J, Elia J, et al. Cell-Surface Marker Signatures for the Isolation of Neural Stem Cells, Glia and Neurons Derived from Human Pluripotent Stem Cells. PLoS ONE. 6(3)(Methodology: Cell culture). View Reference
View All (6) View Less
562337 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.