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BD Pharmingen™ FITC Rat Anti-Mouse IgG3
Clone R40-82 (RUO)
Flow cytometric analysis of intracellular mouse IgG3 on hybridoma cells. Permeabilized cells were stained with FITC Rat Anti-Mouse IgG3 (Cat. No. 553403; solid line histogram) or with FITC Rat IgG1, κ Isotype Control (Cat. No. 554688; dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable hybridoma cells. Flow cytometry was performed on a FACSCalibur™.
Flow cytometric analysis of intracellular mouse IgG3 on hybridoma cells. Permeabilized cells were stained with FITC Rat Anti-Mouse IgG3 (Cat. No. 553403; solid line histogram) or with FITC Rat IgG1, κ Isotype Control (Cat. No. 554688; dashed line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable hybridoma cells. Flow cytometry was performed on a FACSCalibur™.
Product Details
BD Pharmingen™
Ighg3; Immunoglobulin heavy constant gamma 3
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Mouse Pooled Ig
Flow cytometry (Routinely Tested), Intracellular staining (flow cytometry) (Tested During Development)
0.5 mg/ml
AB_394840
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Recommended Assay Procedures
FITC Rat Anti-Mouse IgG3 may be used as a primary or secondary reagent in immunofluorescent staining. FITC Rat Anti-Mouse IgG3 is effective for detection of cell-surface or intracellular Ig by immunofluorescent staining with flow cytometric analysis. Note: intracellular staining is not routinely tested at BD Biosciences Pharmingen. The BD Cytofix/Cytoperm™ Fixation/Permeablization Kit, (Cat. No. 554714) contains the appropriate reagents for intracellular staining.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.1 mg
Cat No.
553141
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Fixation/Permeabilization Kit RUO
Size
250 Tests
Cat No.
554714
FITC Rat IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554688
553403 Rev. 14
Antibody Details
R40-82
The R40-82 antibody specifically recognizes mouse IgG3 of all strains. It does not react with other Ig isotypes.
553403 Rev. 14
Format Details
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
494 nm
518 nm
553403 Rev.14
Citations & References
View product citations for antibody "553403" on CiteAb
Development References (2)
-
Duong BH, Ota T, Aït-Azzouzene D, et al. Peripheral B cell tolerance and function in transgenic mice expressing an IgD superantigen.. J Immunol. 2010; 184(8):4143-58. (Biology). View Reference
-
Kuzin II, Snyder JE, Ugine GD, et al. Tetracyclines inhibit activated B cell function.. Int Immunol. 2001; 13(7):921-31. (Biology). View Reference
553403 Rev. 14
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.