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FITC Mouse Anti-Rat αβ T-Cell Receptor
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FITC Mouse Anti-Rat αβ T-Cell Receptor
Flow cytometric analysis of αβ TCR expression in rat spleen and thymus. Lewis splenocytes were simultaneously stained with PE Mouse Anti-Rat CD3 (Cat. No. 554833, left panels) and FITC Mouse Anti-Rat αβ T-Cell Receptor (Cat. No. 561672/554913; bottom left panel). Lewis thymocytes were stained with FITC Mouse Anti-Rat αβ T-Cell Receptor (bottom right panel) or unstained (top right panel). Fluorescent histograms and two-color contour plots were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
Flow cytometric analysis of αβ TCR expression in rat spleen and thymus. Lewis splenocytes were simultaneously stained with PE Mouse Anti-Rat CD3 (Cat. No. 554833, left panels) and FITC Mouse Anti-Rat αβ T-Cell Receptor (Cat. No. 561672/554913; bottom left panel). Lewis thymocytes were stained with FITC Mouse Anti-Rat αβ T-Cell Receptor (bottom right panel) or unstained (top right panel). Fluorescent histograms and two-color contour plots were derived from gated events with the side and forward light-scattering characteristics of viable cells. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
Product Details
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BD Pharmingen™
TCR α/β: TCR αβ; αβ TCR; alpha beta TCR
Rat (QC Testing)
Mouse BALB/c IgG1, κ
Rat T blasts and rat erythrocytes
Flow cytometry (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
561672 Rev. 2
Antibody Details
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R73

The R73 monoclonal antibody specifically recognizes the αβ T-cell Receptor (TCR) found on most peripheral T lymphocytes, intestinal intraepithelial lymphocytes, and thymocytes. It does not react with γδ TCR-bearing cells. Cross-linked R73 mAb induces T-cell activation and differentiation. In vivo treatment with mAb R73 can suppress immune function of peripheral αβ TCR-expressing T cells, and reduce the severity of experimental autoimmune, transplant rejection, and graft-versus-host responses.

561672 Rev. 2
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
561672 Rev.2
Citations & References
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View product citations for antibody "561672" on CiteAb

Development References (13)

  1. Fangmann J, Schwinzer R, Wonigeit K. Unusual phenotype of intestinal intraepithelial lymphocytes in the rat: predominance of T cell receptor alpha/beta+/CD2- cells and high expression of the RT6 alloantigen. Eur J Immunol. 1991; 21(3):753-760. (Biology). View Reference
  2. Gaede K, Nazet M, Bosse D, Hunig, Heesemann J. Treatment of arthritis in Lewis rats by a monoclonal antibody against alpha beta T cell receptor: differential sensitivity of Yersinia-induced arthritis versus adjuvant arthritis. Clin Immunol Immunopathol. 1995; 77(3):339-348. (Biology). View Reference
  3. Hasegawa T, Tanaka T, Yoshikai Y. The appearance and role of gamma delta T cells in the peritoneal cavity and liver during primary infection with Listeria monocytogenes in rats. Int Immunol. 1992; 4(10):1129-1136. (Biology). View Reference
  4. Heidecke CD, Hancock WW, Jakobs F, et al. alpha/beta-T cell receptor-directed therapy in rat cardiac allograft recipients. Treatment prior to alloantigen exposure prevents sensitization and abrogates accelerated rejection. Transplantation. 1995; 59(1):78-84. (Biology). View Reference
  5. Heidecke CD, Hancock WW, Westerholt S, et al. alpha/beta-T cell receptor-directed therapy in rat allograft recipients. Long-term survival of cardiac allografts after pretreatment with R73 mAb is associated with upregulation of Th2-type cytokines. Transplantation. 1996; 61(6):948-956. (Biology). View Reference
  6. Hunig T, Wallny HJ, Hartley JK, Lawetzky A, Tiefenthaler G. A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes. J Exp Med. 1989; 169(1):73-86. (Immunogen). View Reference
  7. Jung S, Kramer S, Schluesener HJ, Hunig T, Toyka K, Hartung HP. Prevention and therapy of experimental autoimmune neuritis by an antibody against T cell receptors-alpha/beta. J Immunol. 1992; 148(12):3768-3775. (Biology). View Reference
  8. Kiely PD, Thiru S, Oliveira DB. Inflammatory polyarthritis induced by mercuric chloride in the Brown Norway rat. Lab Invest. 1995; 73(2):284-293. (Biology). View Reference
  9. Mitnacht R, Tacke M, Hunig T. Expression of cell interaction molecules by immature rat thymocytes during passage through the CD4+8+ compartment: developmental regulation and induction by T cell receptor engagement of CD2, CD5, CD28, CD11a, CD44 and CD53. Eur J Immunol. 1995; 25(2):328-332. (Biology). View Reference
  10. Pieters RH, Punt P, Bol M, van Dijken JM, Seinen W, Penninks AH. The thymus atrophy inducing organotin compound DBTC stimulates TcR alpha beta-CD3 signalling in immature rat thymocytes. Biochem Biophys Res Commun. 1995; 214(2):552-558. (Biology). View Reference
  11. Wang M, Qu X, Stepkowski SM, Chou TC, Kahan BD. Beneficial effect of graft perfusion with anti-T cell receptor monoclonal antibodies on survival of small bowel allografts in rat recipients treated with brequinar alone or in combination with cyclosporine and sirolimus. Transplantation. 1996; 61(3):458-464. (Biology). View Reference
  12. Yoshino S, Cleland LG, Mayrhofer G, Brown RR, Schwab JH. Prevention of chronic erosive streptococcal cell wall-induced arthritis in rats by treatment with a monoclonal antibody against the T cell antigen receptor alpha beta. J Immunol. 1991; 146(12):4187-4189. (Biology). View Reference
  13. Yoshino S, Cleland LG. Depletion of alpha/beta T cells by a monoclonal antibody against the alpha/beta T cell receptor suppresses established adjuvant arthritis, but not established collagen-induced arthritis in rats. J Exp Med. 1992; 175(4):907-915. (Biology). View Reference
View All (13) View Less
561672 Rev. 2

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