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FITC Mouse Anti-Mouse I-A[k]
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This SKU will be discontinuing Apr 2024. Suggested alternate SKU is [562014] or for additional support, contact your local applications specialist. Contact Us #
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Mouse CWB IgG2a, κ
C3H mouse splenocytes
Flow cytometry (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  5. An isotype control should be used at the same concentration as the antibody of interest.
553540 Rev. 2
Antibody Details
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The 10-3.6 monoclonal antibody specifically recognizes the β chain of the I-A[k] MHC class II alloantigen. It cross-reacts with splenocytes from mice of the H-2[f], H-2[r], and H-2[s] haplotypes, from nonobese diabetic (NOD, H-2[g7]) mice, and with lymph node cells of BN, DA, WRA, and WRC rats. Reactivity with other haplotypes (e.g., b, d, p, q) has not been observed. Long-term in vivo treatment with 10-3.6 mAb has been reported to prevent the spontaneous development of autoimmune insulin-dependent diabetes mellitus (IDDM) in female NOD mice.

553540 Rev. 2
Format Details
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Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
494 nm
518 nm
553540 Rev.2
Citations & References
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Development References (5)

  1. Blankenhorn EP, Symington FW, Cramer DV. Biochemical characterization of Ia antigens encoded by the RT1.B and RT1.D loci in the rat MHC. Immunogenetics. 1983; 17(5):475-484. (Biology). View Reference
  2. Boitard C, Bendelac A, Richard MF, Carnaud C, Bach JF. Prevention of diabetes in nonobese diabetic mice by anti-I-A monoclonal antibodies: transfer of protection by splenic T cells. Proc Natl Acad Sci U S A. 1988; 85(24):9719-9723. (Biology). View Reference
  3. Kupinski JM, Plunkett ML, Freed JH. Assignment of antigenic determinants to separated I-A kappa chains. J Immunol. 1983; 130(5):2277-2281. (Biology). View Reference
  4. Landias D, Beck BN, Buerstedde JM, et al.. The assignment of chain specificities for anti-Ia monoclonal antibodies using L cell transfectants. J Immunol. 1986; 137(9):3002-3005. (Biology). View Reference
  5. Oi VT, Jones PP, Goding JW, Herzenberg LA, Herzenberg LA. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr Top Microbiol Immunol. 1978; 81:115-129. (Immunogen). View Reference
View All (5) View Less
553540 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.