The Ba13 monoclonal antibody specifically recognizes Cell surface glycoprotein CD200 receptor 3 (CD200R3) which is also known as CD200 cell surface glycoprotein receptor-like b (CD200RLb), CD200 receptor-like 3, and OX-2 Receptor 3. CD200R3 is a single-pass type I transmembrane glycoprotein that is encoded by Cd200r3 (CD200 receptor 3) which belongs to the immunoglobulin superfamily (IgSF). It is expressed on basophils and mast cells that are involved in protective immune responses against parasitic infections and in the development of allergic reactions. Cell surface CD200R3 presents as a disulfide-linked dimeric CD200R-like receptor. The cytoplasmic tail region of CD200R3 contains a positively charged lysine residue which allows this receptor to associate and signal through adaptor proteins such as DAP10, DAP12, CD3z, or FcRγ. CD200R3 functions as an activating receptor to regulate IgE-independent immune responses.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.