Flow cytometric analysis of CD196 on mouse splenocytes. BALB/c splenocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse CD196 and FITC Rat Anti-Mouse CD45R/B220 (Cat. No. 553087 or 553088). For data analysis, lymphocytes were selected by their scatter profile, and the positions of the quadrant markers were determined by staining with Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat. No. 557690) and FITC Rat IgG2a, κ Isotype Control (Cat. No. 553929). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Flow cytometric analysis of CD196 on mouse splenocytes. BALB/c splenocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse CD196 and FITC Rat Anti-Mouse CD45R/B220 (Cat. No. 553087 or 553088). For data analysis, lymphocytes were selected by their scatter profile, and the positions of the quadrant markers were determined by staining with Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat. No. 557690) and FITC Rat IgG2a, κ Isotype Control (Cat. No. 553929). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
Product Details
BD Pharmingen™
CCR6, BN-1, CKR-L3, DRY6, KY411, GPR-CY4, STRL22
Mouse (QC Testing)
Rat IgG2a
Mouse CCR6-transfected Rat Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
12458
AB_2228793
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Alexa Fluor® 647 Rat IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
557690
FITC Rat Anti-Mouse CD45R/B220 RUO
Size
0.1 mg
Cat No.
553087
561753 Rev. 6
Antibody Details
140706
The 140706 monoclonal antibody specifically binds to mouse CD196 (CCR6), a CC chemokine receptor that is expressed on B lymphocytes and subsets of T and dendritic cells. The known ligand for CCR6 is CCL20/MIP-3α, and together they regulate the migration and recruitment of antigen-presenting and immunocompetent cells during inflammatory and immunological responses.
561753 Rev. 6
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
561753 Rev.6
Citations & References
View product citations for antibody "561753" on CiteAb
Development References (3)
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Hirota K, Yoshitomi H, Hashimoto M, et al. Preferential recruitment of CCR6-expressing Th17 cells to inflamed joints via CCL20 in rheumatoid arthritis and its animal model. J Exp Med. 2007; 204(12):2803-2812. (Clone-specific: Flow cytometry). View Reference
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Kucharzik T, Hudson JT 3rd, Waikel RL, Martin WD, Williams IR. CCR6 expression distinguishes mouse myeloid and lymphoid dendritic cell subsets: demonstration using a CCR6 EGFP knock-in mouse. Eur J Immunol. 2002; 32(1):104-112. (Biology). View Reference
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Varona R, Villares R, Carramolino L, et al. CCR6-deficient mice have impaired leukocyte homeostasis and altered contact hypersensitivity and delayed-type hypersensitivity responses. J Clin Invest. 2001; 107(6):R37-R45. (Biology). View Reference
561753 Rev. 6
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.