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Multiparameter flow cytometric analysis of CD210a expression on human peripheral blood leucocytes. Human whole blood was stained with either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat. No. 557690; Left Panel) or Alexa Fluor® 647 Rat Anti-Human CD210a (Cat. No. 565255; Right Panel). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Two-parameter flow cytometric contour plots showing the correlated expression of CD210a (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.


BD Pharmingen™ Alexa Fluor® 647 Rat Anti-Human CD210a

Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The 3F9 monoclonal antibody specifically binds to CD210a, which is also known as, Interleukin-10 Receptor subunit alpha (IL-10R subunit alpha/IL-10Rα/IL10RA), or Interleukin-10 receptor subunit 1 (IL-10R1). CD210a is a 90-110 kDa type I transmembrane glycoprotein that belongs to the type II cytokine receptor family. CD210a combines with IL-10 Receptor subunit beta (IL-10Rβ/IL10RB/CDw210b) to form the IL-10 Receptor complex (IL-10Rα/IL-10Rβ) that can bind IL-10 and transduce signals intracellularly through the JAK/STAT pathway. IL-10 can suppress antigen presentation and the expression of proinflammatory type-1 immune responses while promoting type-2 immune responses. CD210a is expressed on T cells, B cells, NK cells, monocyte, macrophages and dendritic cells. Clone 3F9 is specific for human CD210a and its binding to the receptor can be blocked by recombinant human IL-10 (rhIL-10) protein.
Development References (4)
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Callard R, Gearing A. Callard R, Gearing A. The Cytokine Facts Book. San Diego: Academic Press; 1994.
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Carson WE, Lindemann MJ, Baiocchi R, et al. The functional characterization of interleukin-10 receptor expression on human natural killer cells. Blood. 1995; 85(12):3577-3585. (Biology). View Reference
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Liu Y, Wei SH, Ho AS, de Waal Malefyt R, Moore KW. Expression cloning and characterization of a human IL-10 receptor. J Immunol. 1995; 152(4):1821-1829. (Biology). View Reference
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Liu Y, de Waal Malefyt R, Briere F, et al. The EBV IL-10 homologue is a selective agonist with impaired binding to the IL-10 receptor.. J Immunol. 1997; 158(2):604-13. (Immunogen: Blocking, Flow cytometry, Functional assay, Immunoprecipitation, Inhibition). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.