Preparation And Storage
The PE conjugate is supplied as 12.5 μg in 2.0 mL (6.25 μg/mL) of phosphate buffered saline (PBS). The PE-Cy7 conjugate is supplied as 25 μg in 0.5 mL (50 μg/mL) of PBS. The APC conjugate is supplied as 25 μg in 0.5 mL (50 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.
Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
The CD45RO antibody, clone UCHL-1, is derived from hybridization of mouse P3/NS-1/1-Ag4-1 myeloma cells with spleen cells from BALB/c mice immunized with an interleukin-2 (IL-2)–dependent human T-cell line.
The CD45RO antibody recognizes a 180-kilodalton (kDa) isoform of the leucocyte common antigen (LCA). CD45 is a member of the CD45 family that includes CD45, CD45RA, and CD45RB. The CD45 antigen is a protein tyrosine phosphatase.
Development References (10)
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Akbar AN, Terry L, Timms A, Beverley PC, Janossy G. Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. J Immunol. 1988; 140(7):2171-2178. (Biology). View Reference
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D'Arena G, Cascavilla N, Nunziata G, et al. Usefulness of RA and RO isoforms of common leukocyte antigen (CD45) for early distinction between normal and abnormal promyelocytes. Leuk Lymphoma. 2002; 43:1823-1825. (Biology).
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Gamadia LE, Rentenaar RJ, Baars PA, et al. Differentiation of cytomegalovirus-specific CD8(+) T cells in healthy and immunosuppressed virus carriers.. Blood. 2001; 98(3):754-61. (Biology). View Reference
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Ledbetter JA, Tonks NK, Fischer EH, Clark EA. CD45 regulates signal transduction and lymphocyte activation by specific association with receptor molecules on T or B cells. Proc Natl Acad Sci USA. 1988; 85:8628-8632. (Biology).
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Norton AJ, Ramsay AD, Smith SH, Beverley PC, Isaacson PG. Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues. J Clin Pathol. 1986; 39(4):399-405. (Biology). View Reference
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Ostrowski SR, Gerstoff J, Pederson BK, Ullum H. A low level of CD4+CD28+ T cells is an independent predictor of high mortality in human immunodeficiency virus type 1–infected patients. J Infec Dis. 2003; 187:1726-1734. (Biology).
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Picker LJ, Treer JR, Ferguson-Darnell B, Collins PA, Buck D, Terstappen LW. Control of lymphocyte recirculation in man. I. Differential regulation of the peripheral lymph node homing receptor L-selectin on T cells during the virgin to memory cell transition. J Immunol. 1993; 150(3):1105-1121. (Biology). View Reference
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Smith SH, Brown MH, Rowe D, Callard RE, Beverley PC. Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL-1. Immunology. 1986; 58:63-70. (Biology).
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Streuli M, Morimoto C, Schrieber M, Schlossman SF, Saito H. Characterization of CD45 and CD45R monoclonal antibodies using transfected mouse cell lines that express individual human leukocyte common antigens. J Immunol. 1988; 141(11):3910-3914. (Biology). View Reference
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Tonks NK, Charbonneau H, Diltz CD, Fischer EH, Walsh KA. Demonstration that the leukocyte common antigen CD45 is a protein tyrosine phosphatase. Biochemistry. 1988; 27:8695-8701. (Biology).
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