-
Your selected country is
Belgium
- Change country/language
Old Browser
This page has been recently translated and is available in French now.
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
The FITC and PE conjugates are each supplied as 1 μg in 1.0 mL (1 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.
Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
The Anti–bcl-2 antibody, clone 100, is derived from the hybridization of Sp2/0-Ag14 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with the bcl-2α peptide (amino acids 41-54). The Anti–bcl-2 antibody recognizes a 26-kilodalton (kDa) protein encoded by the 230 kb bcl-2 protooncogene. The protein is a regulator of the apoptotic process, and elevated levels can provide resistance to cell death. The bcl-2 protein is expressed in the interior of cells, frequently localized to the mitochondrial membrane.
Development References (11)
-
Aiello A, Delia D, Borrello MG, et al. Flow cytometric detection of the mitochondrial BCL-2 protein in normal and neoplastic human lymphoid cells. Cytometry. 1992; 13(5):502-509. (Biology). View Reference
-
Campos L, Rouault J-P, Sabido O, et al. High expression of bcl-2 protein in acute myeloid leukemia cells is associated with poor response to chemotherapy. Blood. 1993; 81:3091-3096. (Biology).
-
Cleary ML, Smith SD, Sklar J. Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation. Cell. 1986; 47:19-28. (Biology).
-
Coustan-Smith E, Kitanaka A, Pui C-H, et al. Clinical relevance of bcl-2 overexpression in childhood acute lymphoblastic leukemia. Blood. 1996; 87:1140-1146. (Biology).
-
DiGiuseppe JA, LeBeau P, Augenbraun J, Borowitz MJ. Multiparameter flow-cytometric analysis of bcl-2 and fas expression in normal and neoplastic hematopoiesis. Am J Clin Pathol. 1996; 106:345-351. (Biology).
-
Hermine O, Haioun C, Lepage E, et al. Prognostic significance of bcl-2 protein expression in aggressive non-Hodgkin's lymphoma. Blood. 1996; 87:265-272. (Biology).
-
Krajewski S, Tanaka S, Takayama S, Schibler MJ, Fenton W, Reed JC. Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes. Cancer Res. 1993; 53(19):4701-4714. (Biology). View Reference
-
Pezzella F, Tse AG, Cordell JL, Pulford KA, Gatter KC, Mason DY. Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation. Am J Pathol. 1990; 137(2):225-232. (Biology). View Reference
-
Reed JC. Double identity for proteins of the bcl-2 family. Nature. 1997; 387:773-776. (Biology).
-
Tsujimoto Y, Croce CM. Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. Proc Natl Acad Sci U S A. 1986; 83(14):5214-5218. (Biology). View Reference
-
Zamai L, Canonico B, Gritzapis A, et al. Intracellular detection of Bcl-2 and p53 proteins by flow cytometry: comparison of monoclonal antibodies and sample preparation protocols. J Biol Regul Homeost Agents. 2002; 16:289–302. (Biology).
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Although not required, these products are manufactured in accordance with Good Manufacturing Practices.