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FITC Mouse Anti-Human CD103
Product Details
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ITGAE; Integrin alpha-E; ITAE; HML-1 antigen; Mucosal lymphocyte 1; HUMINAE
Mouse BALB/c IgG1, κ
Human MAPS16 T Cell Line
Flow cytometry
25 μg/mL
20 μL
V A067, BP047, S256
Phosphate buffered saline with gelatin and sodium azide.

Preparation And Storage

Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340945 Rev. 1
Antibody Details
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The CD103 antibody, clone Ber-ACT8, is derived using standard cell hybridization procedures using spleen cells from BALB/c mice immunized with the HTLV-1–positive T-cell line, MAPS16.

The CD103 antibody recognizes the αE subunit of integrin αEβ

, an integrin also known as the human mucosal lymphocyte (HML) antigen 1, but not α, another member of the βintegrin subfamily. Integrin αEβis a trimeric protein complex of three components, 105 kilodaltons (kDa) (β), 150 kDa, and 25 kDa. The CD103 antibody recognizes an epitope localized on the 150-kDa chain.

340945 Rev. 1
Format Details
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Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
494 nm
518 nm
340945 Rev.1
Citations & References
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View product citations for antibody "340945" on CiteAb

Development References (14)

  1. Brew R, West DC, Burthem J, Christmas SE. Expression of the human mucosal lymphocyte antigen, HML-1, by T cells activated with mitogen or specific antigen in vitro. Scan J Immunol. 1995; 41:553–562. (Biology).
  2. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  3. Cerf-Benussan N, Jarry A, Brousse N, Lisowska-Grospierre B, Guy-Grand D, Griscelli C. A monoclonal antibody (HML-1) defining a novel membrane molecule present on human intestinal lymphocytes. Eur J Immunol. 1987; 17:1279–1285. (Biology).
  4. Clinical and Laboratory Standards Institute. 2005. (Biology).
  5. Falini B, Pileri SA, Flenghi L, et al. Selection of a panel of monoclonal antibodies for monitoring residual disease in peripheral blood and bone marrow of interferon-treated hairy cell leukaemia patients. Br J Haematol. 1990; 76:460–468. (Biology).
  6. Hadley GA, Rostapshova EA, Gomolka DM, et al. Regulation of the epithelial cell-specific integrin, CD103, by human CD8+ cytolytic T lymphocytes. Transplantation. 1999; 67:1418–1425. (Biology).
  7. Hassan IB, Hagberg H, Sundström C. Immunophenotype of hairy-cell leukemia. Eur J Haematol. 1990; 45:172-176. (Biology).
  8. Kruschwitz M, Fritzsche G, Schwarting R, et al. Ber-ACT8: new monoclonal antibody to the mucosa lymphocyte antigen. J Clin Pathol. 1991; 44(8):636-645. (Biology). View Reference
  9. Lohmeyer J, Friedrish J, Grimminger F, et al. Expression of mucosa-related integrin αEβ7 on alveolar T cells in interstitial lung diseases. Clin Exp Immunol. 1999; 116:340-346. (Biology).
  10. Micklem KJ, Dong Y, Willis A, et al. HML-1 antigen on mucosa-associated T cells, activated cells, and hairy leukemic cells is a new integrin containing the β7 subunit. Am J Pathol. 1996; 3:30-36. (Biology).
  11. Moldenhauer G, Mielke B, Dörken B, Schwartz-Albiez R, Möller P. Identity of HML-1 antigen on intestinal intraepithelial T cells and of B-ly7 antigen on hairy cell leukaemia. Scand J Immunol. 1990; 32:77–82. (Biology).
  12. Möller P, Mielke B, Moldenhauer G. Monoclonal antibody HML-1, a marker for intraepithelial T cells and lymphomas derived thereof, also recognizes hairy cell leukemia and some B-cell lymphomas. Am J Pathol. 1990; 136:509–512. (Biology).
  13. Parker CM, Cepek KL, Russell GJ, et al. A family of beta 7 integrins on human mucosal lymphocytes. Proc Natl Acad Sci U S A. 1992; 89(5):1924-1928. (Biology). View Reference
  14. Rihs S, Walker C, Virchow JC Jr, et al. Differential expression of α E β 7 integrins on bronchoalveolar lavage T lymphocyte subsets: regulation by α 4 β 1-integrin crosslinking and TGF-β. Am J Respir Cell Mol Biol. 1996; 15:600–610. (Biology).
View All (14) View Less
340945 Rev. 1

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