BD FastImmune™ Antibody Cocktail (IFN-γ FITC, CD69 PE, CD4 PerCP-Cy™5.5)
Description
Anti-Hu–IFN-γ, clone 25723.11, is derived from the hybridization of P3X-63-Ag8.653 mouse myeloma cells with lymph node cells from BALB/c mice immunized with recombinant human IFN-γ. CD69, clone L78, is derived from hybridization of Sp2/0-Ag14 mouse myeloma cells with lymph node cells from BALB/c mice immunized with a CD8+ alloantigen-directed cytotoxic T-lymphocyte (CTL) cell line. CD4, clone SK3, is derived from hybridization of NS-1mouse myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes. Anti-Human Interferon-γ (Anti-Hu–IFN-γ) recognizes a 20- to 25-kilodalton (kDa) glycoprotein. CD69 recognizes a very early human activation antigen. The CD69 antigen is a surface homodimer formed by the association of 28-kDa and 32-kDa chains that are held together by disulfide bridges. The CD4 antigen, with a molecular weight of 55 kDa, is present on T-helper/inducer lymphocytes and monocytes.
Preparation And Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
| Description | Clone | Isotype | EntrezGene ID |
|---|---|---|---|
| N/A | N/A | | IgG2b, ,IgG1, κ,IgG1, κ | N/A |
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
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