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Anti-Human HLA-DR PE/Monocyte PerCP-Cy™5.5

Anti-Human HLA-DR PE/Monocyte PerCP-Cy™5.5

(RUO (GMP))
Product Details
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BD Quantibrite™
Human
Flow cytometry
RUO (GMP)
Buffered gelatin solution with an HLA-DR stabilizing reagent and 0.1% sodium azide.


Description

Anti–HLA-DR, clone L243, is derived from the hybridization of mouse NS-1/1-Ag4 myeloma cells with spleen cells from BALB/c mice immunized with the human lymphoblastoid B-cell line RPMI 8866.

CD14, clone MφP9, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with peripheral blood monocytes from a patient with rheumatoid arthritis.

Anti–HLA-DR recognizes a human class II major histocompatibility complex (MHC) antigen. The antigen is a transmembrane glycoprotein composed of α and β subunits that have molecular weights of 36 and 27 kDaltons (kDa), respectively. Anti–HLA-DR reacts with a nonpolymorphic HLA-DR epitope and does not cross-react with HLA-DQ or HLA-DP molecules.

Anti-Monocyte PerCP-Cy5.5 reagent recognizes monocytes using two discrete specificities. This reagent includes CD14, a human monocyte/macrophage antigen, Mr 55 kDa, that recognizes the majority of the monocyte population. In addition, Anti-Monocyte PerCP-Cy5.5 recognizes FcR1 (CD64) by taking advantage of the unique recognition of CD64 by cyanine dyes. Anti-Monocyte PerCP-Cy5.5 is specially formulated to take advantage of this property of the cyanine dye to ensure detection of all monocytes (CD14 brightly positive and weakly positive) in a sample.

Preparation And Storage

The Anti–HLA-DR/Anti-Monocyte PerCP-Cy5.5 reagent is supplied as a combination of Anti–HLA-DR PE (≥95% 1:1 PE:mAb ratio) and Anti-Monocyte PerCP-Cy5.5 in a buffered gelatin solution containing an HLA-DR stabilizing reagent and 0.1% sodium azide. Store vials in the dark at 2° to 8°C. Do not freeze QuantiBRITE reagents; protect them from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340827 Rev. 1
Components
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Description Clone Isotype EntrezGene ID
HLA-DR 1 1 RPE N/A N/A N/A
CD14 PerCP-Cy5.5 N/A N/A N/A
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Citations & References
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Development References (22)

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  8. Herrmann F, Komischke B, Odenwald E, Ludwig W. Use of monoclonal antibodies as a diagnostic tool in human leukemia, I: Acute myeloid leukemia and acute phase of chronic myeloid leukemia. Blut. 1983; 47:157-163. (Biology).
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  17. Wakefield CH, Carey PD, Foulds S, Monson JRT, Guillou PJ. Changes in major histocompatibility complex class II expression in monocytes and T cells of patients developing infection after surgery. Brit J Surg. 1993; 80:205-209. (Biology).
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