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BD Pharmingen™ Purified Mouse anti-p130Cas (pY249)
Clone J169-757.12.2 (RUO)
Western blot analysis of p130Cas (pY249) in human Burkitt’s lymphoma. Lysates from control (left panel) and hydrogen peroxide-activated (right panel) Ramos cells were probed with purified mouse anti-p130Cas (pY249) monoclonal antibody) at concentrations of 0.125 (lanes 1 and 4), 0.0625 (lanes 2 and 5), and 0.0312 µg/ml (lanes 3 and 6). p130Cas (pY249) is identified as a band of 130 kDa in the treated cells.
Western blot analysis of p130Cas (pY249) in human Burkitt’s lymphoma. Lysates from control (left panel) and hydrogen peroxide-activated (right panel) Ramos cells were probed with purified mouse anti-p130Cas (pY249) monoclonal antibody) at concentrations of 0.125 (lanes 1 and 4), 0.0625 (lanes 2 and 5), and 0.0312 µg/ml (lanes 3 and 6). p130Cas (pY249) is identified as a band of 130 kDa in the treated cells.
Product Details
BD Pharmingen™
Human (QC Testing)
Mouse IgG2b, κ
Phosphorylated Human p130Cas
Western blot (Routinely Tested)
130 kDa
0.5 mg/ml
AB_647286
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
558401 Rev. 2
Antibody Details
J169-757.12.2
p47v-crk (v-Crk) is the product of a transforming gene, v-crk, that was isolated from avian sarcoma viruses. The v-Crk protein is a fusion product of viral Gag protein and a part of cellular Crk that includes SH2 and SH3 domains. v-Crk-induced transformation increases tyrosine phosphorylation of several cellular proteins, including p130Cas (CRK-associated substrate). The p130Cas is tightly associated with v-Crk via the SH2 domain of v-Crk. Tyrosine phosphorylation of p130Cas occurs in conjunction with cellular transformation in cells that express v-Src or v-Crk. This phosphorylation leads to a change in p130Cas localization from the cytoplasm to the cell membrane and, possibly, to the nucleus. Since p130Cas also associates with v-Src, it may be a v-Src substrate. Several phosphorylation sites have been described in p130Cas upon Fibroblast Growth Factor stimulation, and phosphorylated tyrosine (Y249) might function as a binding site for the Crk-adaptor molecule.
The J169-757.12.2 monoclonal antibody recognizes the phosphorylated Y249 of human p130Cas. The orthologous phosphorylation sites in mouse and rat p130Cas are Y253 and Y347, respectively.
558401 Rev. 2
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
558401 Rev.2
Citations & References
View product citations for antibody "558401" on CiteAb
Development References (2)
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Goldberg GS, Alexander DB, Pellicena P, Zhang Z-Y, Tsuda H, Miller WT. Src phosphorylates Cas on tyrosine 253 to promote migration of transformed cells. J Biol Chem. 2003; 278(47):46533-46540. (Biology).
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Hinsby AM, Olsen JV, Bennett KL, Mann M. Signaling initiated by overexpression of the fibroblast growth factor receptor-1 investigated by mass spectrometry. Mol Cell Proteomics. 2003; 2(1):29-36. (Biology).
558401 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.