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BD Transduction Laboratories™ Integrins Sampler Kit
(RUO)Each antibody has been tested on an appropriate positive control lysate (see table).
IP: nat = native conditions, den = denaturing conditions Dilutions are recommended based on western blotting on the indicated positive control This kit includes 10 µg of each antibody listed at a concentration of 250 µg/ml. No substitutions allowed.
BD Transduction Laboratories™ Integrins Sampler Kit
BD Transduction Laboratories™ Integrins Sampler Kit
Integrins Sampler Kit
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Description
Cell adhesion to extracellular matrix (ECM) components has been reported to be mediated by the integrins. These proteins link the ECM to the intracellular cytoskeletal network and to multiple intracellular signaling pathways whose activation is important for cell survival, growth, and differentiation. Integrins contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α and 8 β subunits have been identified and these proteins can heterodimerize to form 22 different receptors. Some integrins interact with only one specific ligand. It is more common that a specific integrin recognizes multiple ligands. There is a high degree of redundancy, so that integrins which interact identically with a particular ligand do not necessarily induce the same cellular response. Either one or both subunits directly interact with a number of proximal intracellular signaling components such as cytoskeletal proteins (talin, fibronectin, α-actinin), adaptor molecules (paxillin, RACK1, p130Cas), Ca2+ binding proteins (CIB and calreticulin), and protein kinases (FAK and ILK). Such interactions initiate multiple pathways that signal in the traditional "outside-in" signaling process. When bound by ligand, they may form signaling connections with intracellular components or may remain detached from the cytoskeleton and signaling pathways. In the absence of intracellular signaling, integrin ligation can promote the assembly and organization of the intercellular ECM.
These antibodies are routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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