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Western blot analysis of Sec8 on a MDCK cell lysate (Canine kidney; ATCC CCL-34). Lane 1: 1:1000, lane 2: 1: 2000, lane 3: 1: 4000 dilution of the mouse anti-Sec8 antibody.
Immunofluorescence of AN3 CA cells (Human endometrial adenocarcinoma; ATCC HTB-111).
BD Transduction Laboratories™ Purified Mouse Anti-Sec8
BD Transduction Laboratories™ Purified Mouse Anti-Sec8
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
Signal transmission between neurons is regulated by the release of neurotransmitters at the synapse. This process, which is controlled by a complex pathway of membrane trafficking in the presynaptic nerve terminal, leads to membrane fusion and neurotransmitter secretion. Sec8 is a hydrophilic protein of 975 amino acids that is highly expressed in brain and kidney. It is homologus with the yeast secretory protein, Sec8p. In yeast, Sec8 is essential for the Golgi-to-plasma membrane traffic of proteins during constitutive secretion. In rat brain, Sec8 colocalizes with Rab3 and Syntaxin-1a, two important proteins that regulate the fusion of the synaptic vesicle to the plasma membrane. The primary structure of Sec8, a coiled-coil domain (residues 34-99), is commonly found in other proteins important in secretory pathways.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (4)
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Charron AJ, Nakamura S, Bacallao R, Wandinger-Ness A. Compromised cytoarchitecture and polarized trafficking in autosomal dominant polycystic kidney disease cells. J Cell Biol. 2000; 149(1):111-124. (Biology: Immunofluorescence, Western blot). View Reference
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Duncan RR, Don-Wauchope AC, Tapechum S, Shipston MJ, Chow RH, Estibeiro P. High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells. Biochem J. 1999; 342(Pt 3):497-501. (Biology: Western blot). View Reference
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Hazuka CD, Hsu SC, Scheller RH. Characterization of a cDNA encoding a subunit of the rat brain rsec6/8 complex. Gene. 1997; 187(1):67-73. (Biology). View Reference
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Ting AE, Hazuka CD, Hsu SC. rSec6 and rSec8, mammalian homologs of yeast proteins essential for secretion. Proc Natl Acad Sci U S A. 1995; 92(21):9613-9617. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.