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Western blot analysis of Annexin II on a A431 lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of the anti- Annexin II antibody.
Immunofluorescence staining of human fibroblasts.
BD Transduction Laboratories™ Purified Mouse Anti-Annexin II
BD Transduction Laboratories™ Purified Mouse Anti-Annexin II
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
Annexin II (p36), originally discovered as a substrate for the src oncogene, is one of the most studied members of the annexin family of calcium-dependent phospholipid-binding proteins. It exists in cells as either a monomer or a heterotetramer complexed with p11, an S100-related protein. In vitro, it has been found that unphosphorylated annexin II is capable of causing aggregation of chromaffin granules, and, upon phosphorylation, induces fusion of the granules' membranes. A similiar phenomenon may occur in the final steps of exocytosis after cytoplasmic annexin translocates to the subplasmalemmal region and is phosphorylated by protein kinase C. However, other studies refute annexin II's involvement in exocytosis, and a conclusive role for annexin II in membrane trafficking remains debated.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (5)
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Chasserot-Golaz S, Vitale N, Sagot I, et al. Annexin II in exocytosis: catecholamine secretion requires the translocation of p36 to the subplasmalemmal region in chromaffin cells. J Cell Biol. 1996; 133(6):1217-1236. (Biology). View Reference
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Choi KS, Fitzpatrick SL, Filipenko NR, et al. Regulation of plasmin-dependent fibrin clot lysis by annexin II heterotetramer. J Biol Chem. 2001; 276(27):25212-25221. (Biology: Immunohistochemistry). View Reference
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Hansen MD, Ehrlich JS, Nelson WJ. Molecular mechanism for orienting membrane and actin dynamics to nascent cell-cell contacts in epithelial cells. J Cell Biol. 2002; 277(47):45371-45376. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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Jacovina AT, Zhong F, Khazanova E, Lev E, Deora AB, Hajjar KA. Neuritogenesis and the nerve growth factor-induced differentiation of PC-12 cells requires annexin II-mediated plasmin generation. J Biol Chem. 2001; 276(52):49350-49358. (Biology: Immunofluorescence, Western blot). View Reference
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Kwon M, Caplan JF, Filipenko NR, et al. Identification of annexin II heterotetramer as a plasmin reductase. J Biol Chem. 2002; 277(13):10903-10911. (Biology: Flow cytometry). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.