Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Netherlands (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified Rat Anti-Mouse IgG2a
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
      Pooled mouse Ig
      ELISA Capture (Routinely Tested)
      0.5 mg/ml
      380793
      AB_394864
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      For the sandwich IgG2a ELISA, R11-89 mAb is optimal for capture with biotin-, AKP-, or HRP-conjugated anti-mouse IgG2a R19-15 mAb (Cat. No. 553388, 553389, or 553391, respectively) for detection. This pair of anti-mouse IgG2a mAbs can effectively quantitate mouse IgG2a of these mouse Igh-C haplotypes, in order of sensitivity from highest to lowest: e, a, j, and b.

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      Show More blue down arrow Show Less blue up arrow
      553446 Rev. 6
      Antibody Details
      Down Arrow Up Arrow
      R11-89

      The R11-89 antibody recognizes mouse IgG2a, with strong reactivity to the Igh-1[a] allotype and weaker reactivity to Igh-1[b]. It does not react with other Ig isotypes. Molecular genetic analyses suggest that the Igh-I[b] allele, which encodes IgG2a[b], is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.

      553446 Rev. 6
      Format Details
      Down Arrow Up Arrow
      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      553446 Rev.6
      Citations & References
      Down Arrow Up Arrow

      Development References (2)

      1. Martin RM, Silva A, Lew AM. The Igh-1 sequence of the non-obese diabetic (NOD) mouse assigns it to the IgG2c isotype. Immunogenetics. 1997; 46(2):167-168. (Biology). View Reference
      2. Morgado MG, Cam P, Gris-Liebe C, Cazenave PA, Jouvin-Marche E. Further evidence that BALB/c and C57BL/6 gamma 2a genes originate from two distinct isotypes. EMBO J. 1989; 8(11):3245-3251. (Biology). View Reference
      553446 Rev. 6

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback