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      Purified Mouse Anti-Human MCP-1
      Product Details
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      BD Pharmingen™
      Human (QC Testing)
      Mouse IgG1, κ
      E-coli-expressed recombinant human MCP-1
      ELISA Capture (Routinely Tested)
      0.5 mg/ml
      AB_395676
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Recommended Assay Procedures

      ELISA Capture: The purified 10F7 antibody is useful as a capture antibody for a sandwich ELISA for measuring human MCP-1 protein levels. The purified 10F7 antibody can be paired with the biotinylated anti-human MCP-1 monoclonal antibody 5D3-F7 (Cat. No. 554664) as the detection antibody with recombinant human MCP-1 (Cat. No. 554620) as the standard. Purified 10F7 antibody should be titrated 2-6 µg/ml to determine the optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of human MCP-1 ranging from ~5000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the chapter on ELISA in the Immune Function Handbook, which is posted on our website at www.bdbiosciences.com.

              Note 1: This ELISA pair shows no cross-reactivity with any of the following chemokines tested (e.g., Human eotaxin, MCP-2, MCP-3, MCP-4, mouse MCP-1 and rat MCP-1).

              Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum samples. BD OptEIA™ ELISA Set Cat. No. 555179 and Kit 559017 are specially-formulated for serum cytokine measurement.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      555055 Rev. 1
      Antibody Details
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      10F7

      The 10F7 antibody reacts with human monocyte chemoattractant protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF). The immunogen used to generate the 10F7 hybridoma was E. coli-expressed recombinant human MCP-1.

      This antibody is routinely tested by ELISA Capture. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      555055 Rev. 1
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      555055 Rev.1
      Citations & References
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      View product citations for antibody "555055" on CiteAb

      Development References (2)

      1. Matsushima K, Oppenheim JJ. Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF. Cytokine. 1989; 1(1):2-13. (Biology). View Reference
      2. Yoshimura T, Yuhki N, Moore SK, Appella E, Lerman MI, Leonard EJ. Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning, expression in mitogen-stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE. FEBS Lett. 1989; 244(2):487-493. (Biology). View Reference
      555055 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.