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      RY586 Mouse Anti-Mouse TCR DO-11.10 Clonotypic
      Product Details
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      BD OptiBuild™
      DO-11.10 clonotypic TCR
      Mouse (Tested in Development)
      Mouse BALB.B x AKR IgG2a, κ
      DO-11.10 T hybridoma cells
      Flow cytometry (Qualified)
      0.2 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Researchers should determine the optimal concentration of this reagent for their individual applications.
      2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. CF™ is a trademark of Biotium, Inc.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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      753169 Rev. 1
      Antibody Details
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      KJ1-26

      The KJ1-26 monoclonal antibody specifically reacts with the DO-11.10 Clonotypic T-cell Receptor (TCR) of the BALB/c-derived DO-11.10 T-cell hybridoma and T lymphocytes from the DO-11.10 transgenic mouse (TgN[DO-11.10]10Loh). The DO-11.10 TCR, an 80-90-kDa (non-reduced) or 40-44-kDa (reduced) protein, is specific for the chicken OVA(323-339)/I-A[d] complex. The DO-11.10 T-cell hybridoma also responds strongly to chicken OVA/I-A[b] and jungle fowl OVA/I-A[d] and weakly to turkey OVA/I-A[d] and I-A[b]. The DO-11.10 mouse model is valuable for studies of T-cell immigration, immunoregulation, development, activation, and function. The KJ1-26 mAb was shown to block the antigen responses of the DO-11.10 T-cell hybridoma in vitro.

      753169 Rev. 1
      Format Details
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      RY586
      The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
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      RY586
      Yellow-Green 561 nm
      564 nm
      586 nm
      753169 Rev.1
      Citations & References
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      View product citations for antibody "753169" on CiteAb

      Development References (7)

      1. Egan RM, Yorkey C, Black R, Loh WK, Stevens JL, Woodward JG. Peptide-specific T cell clonal expansion in vivo following immunization in the eye, an immune-privileged site. J Immunol. 1996; 157(6):2262-2271. (Biology). View Reference
      2. Garside P, Ingulli E, Merica RR, Johnson JG, Noelle RJ, Jenkins MK. Visualization of specific B and T lymphocyte interactions in the lymph node. Science. 1998; 281(5373):96-99. (Clone-specific: Immunohistochemistry). View Reference
      3. Haskins K, Kubo R, White J, Pigeon M, Kappler J, Marrack P. The major histocompatibility complex-restricted antigen receptor on T cells. I. Isolation with a monoclonal antibody. J Exp Med. 1983; 157(4):1149-1169. (Immunogen: Blocking, Immunoprecipitation, Inhibition, Neutralization, Radioimmunoassay). View Reference
      4. Jackson Laboratories. Jax® Mice and Services. Available: http://jaxmice.jax.org 2001, November.
      5. Lee WT, Cole-Calkins J, Street NE. Memory T cell development in the absence of specific antigen priming. J Immunol. 1996; 157(12):5300-5307. (Biology). View Reference
      6. Marrack P, Shimonkevitz R, Hannum C, Haskins K, Kappler J. The major histocompatibility complex-restricted antigen receptor on T cells. IV. An antiidiotypic antibody predicts both antigen and I-specificity. J Exp Med. 1983; 158(5):1635-1646. (Clone-specific: Blocking, ELISA, Immunoprecipitation). View Reference
      7. Murphy KM, Heimberger AB, Loh DY. Induction by antigen of intrathymic apoptosis of CD4+CD8+TCRlo thymocytes in vivo. Science. 1990; 250(4988):1720-1723. (Biology). View Reference
      View All (7) View Less
      753169 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.