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PE Rat Anti-Mouse Ig κ Light Chain
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
Mouse IgG2b κ secreted by MPC-11 plasmacytoma
Flow cytometry (Routinely Tested)
0.2 mg/ml
16071
AB_397384
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

This antibody conjugate has been tested by immunofluorescent staining (≤ 1 µg/million cells) with flow cytometric analysis to assure specificity and reactivity. PE-conjugated 187.1 mAb may be used as a primary or secondary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic Ig, κ light chain, we recommend FITC-conjugated mAb 187.1 (Cat. No. 550003).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
559940 Rev. 6
Antibody Details
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187.1

The 187.1 monoclonal antibody specifically binds to kappa light chains of mouse immunoglobulins. The 187.1 antibody does not react with mouse λ1 or λ2 immunoglobulin lights chains or mouse immunoglobulin heavy chains.

559940 Rev. 6
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
559940 Rev.6
Citations & References
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Development References (1)

  1. Yelton DE, Desaymard C, Scharff MD. Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies. Hybridoma. 1981; 1(1):5-11. (Immunogen). View Reference
559940 Rev. 6

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.