Multi-color flow cytometric analysis of DCAR2 (Clec4b1) expression on mouse leucocytes and transfectants. Left and Middle Panels: Bone marrow cells from C57BL/6 mice were incubated in the presence of Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141 or 553142) and were stained simultaneously with APC Hamster Anti-Mouse CD11c (Cat. No. 550261 or 561119) and FITC Rat Anti-Mouse I-A/I-E antibody (Cat. No. 553623 or 562009) to gate on conventional dendritic cells (cDCs) as shown in the left panel. At the same time, the samples of the bone marrow cells were also stained with either PE Rat Anti-Mouse DCAR2 (Clec4b1) (Cat. No. 566882) or PE Rat IgG2b, κ Isotype Control (Cat. No. 553989) at 0.5 µg/test. The overlayed plots of the middle panel were derived from gated cDCs showing expression of DCAR2 (Clec4b1) or Ig Isotype Control staining (solid and dashed line histograms, respectively). Right Panel: Non-transfected (dotted line histogram) and mouse DCAR2-transfected (solid line histogram) 293F cells were surface stained with PE Rat Anti-Mouse DCAR2 (Clec4b1) antibody (Cat. No. 566882) at 0.5 µg/test. Histogram plots showing expression of DCAR2 (Clec4b1) on non-transfected or Mouse DCAR2-transfected 293F cells were derived from gated events with forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
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Multi-color flow cytometric analysis of DCAR2 (Clec4b1) expression on mouse leucocytes and transfectants. Left and Middle Panels: Bone marrow cells from C57BL/6 mice were incubated in the presence of Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141 or 553142) and were stained simultaneously with APC Hamster Anti-Mouse CD11c (Cat. No. 550261 or 561119) and FITC Rat Anti-Mouse I-A/I-E antibody (Cat. No. 553623 or 562009) to gate on conventional dendritic cells (cDCs) as shown in the left panel. At the same time, the samples of the bone marrow cells were also stained with either PE Rat Anti-Mouse DCAR2 (Clec4b1) (Cat. No. 566882) or PE Rat IgG2b, κ Isotype Control (Cat. No. 553989) at 0.5 µg/test. The overlayed plots of the middle panel were derived from gated cDCs showing expression of DCAR2 (Clec4b1) or Ig Isotype Control staining (solid and dashed line histograms, respectively). Right Panel: Non-transfected (dotted line histogram) and mouse DCAR2-transfected (solid line histogram) 293F cells were surface stained with PE Rat Anti-Mouse DCAR2 (Clec4b1) antibody (Cat. No. 566882) at 0.5 µg/test. Histogram plots showing expression of DCAR2 (Clec4b1) on non-transfected or Mouse DCAR2-transfected 293F cells were derived from gated events with forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Product Details
BD Pharmingen™
C-type lectin domain family 4, member b1; Clec4b; DCAR; DCARbeta; Dcar2
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2b, κ
Mouse DCAR2 extracellular domain- Transfected Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2869933
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.1 mg
Cat No.
553141
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO
Size
0.5 mg
Cat No.
553142
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
APC Hamster Anti-Mouse CD11c RUO
Size
0.1 mg
Cat No.
550261
APC Hamster Anti-Mouse CD11c RUO
Size
25 µg
Cat No.
561119
FITC Rat Anti-Mouse I-A/I-E RUO
Size
0.5 mg
Cat No.
553623
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566882 Rev. 1
Antibody Details
7D2D3
The 7D2D3 monoclonal antibody specifically recognizes mouse Dendritic Cell Activating Receptor 2 (DCAR2), a C-type lectin encoded by the Clec4b1 gene. DCAR2 (Clec4b1) is a member of the dendritic cell immunoreceptor (DCIR) family, which contains four dendritic cell inhibitory receptors (DCIR1-4) and two activating receptors (DCAR1, 2). The extracellular region of DCAR2 (Clec4b1) has one C-type lectin carbohydrate recognition domain (CRD), that is highly similar to that of DCIR1, followed by a transmembrane region. Instead of a cytoplasmic Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM) that can transmit inhibitory signals, DCAR2 associates with the Immunoreceptor Tyrosine-based Activating Motif (ITAM)-containing adaptor molecule, Fc Receptor γ (FcRγ). DCAR2 (Clec4b1) is expressed on restricted subsets of conventional dendritic cells in bone marrow and skin-draining lymph nodes. C-type lectins bind to carbohydrates in a calcium-dependent manner, and this receptor may be involved in the regulation of immune responses to bacterial infections.
566882 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566882 Rev.1
Citations & References
View product citations for antibody "566882" on CiteAb
Development References (4)
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Hsu Y, Okada R, Nishimura T, Kawasaki N, Yamamoto K, Matsumoto N. DCIR3 and DCIR4 are co-expressed on inflammatory and patrolling monocytes. Biochem Biophys Res Commun.. 2017; 494(3-4):440-445. (Clone-specific: Flow cytometry). View Reference
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Kanazawa N, Tashiro K, Inaba K, Miyachi Y. Dendritic cell immunoactivating receptor, a novel C-type lectin immunoreceptor, acts as an activating receptor through association with Fc receptor gamma chain.. J Biol Chem. 2003; 278(35):32645-52. (Biology). View Reference
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Kishimoto A, Watanabe M, Terauchi K, et al. Ubiquitous versus restricted expression of the two mouse dendritic cell C-type lectin receptors, DCIR1 and DCAR2, among myeloid cells.. Biochem Biophys Res Commun. 2015; 467(2):383-8. (Immunogen: Flow cytometry). View Reference
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Toyonaga K, Torigoe S, Motomura Y, et al. C-Type Lectin Receptor DCAR Recognizes Mycobacterial Phosphatidyl-Inositol Mannosides to Promote a Th1 Response during Infection. Immunity. 2016; 45(6):1245-1257. (Biology). View Reference
566882 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.