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PE Mouse anti-Sox2
PE Mouse anti-Sox2

Flow cytometric analysis of PE Mouse anti-Sox2 in H9 cells. H9 human embryonic stem (ES) cells (WiCell, Madison, WI) were fixed (BD Cytofix™ buffer, Cat. No. 554655) for 10 minutes at 37°C, permeabilized with BD™ Phosflow Perm Buffer III (Cat. No. 558050) on ice for 30 minutes, and then stained with either PE Mouse anti-Sox2 (solid line) or PE Mouse IgG2a Isotype Control (dashed line).  Any of the three BD™ Phosflow permeabilization buffers may be used with this antibody: BD™ Phosflow Perm/Wash Buffer I (Cat. No. 557885), BD™ Phosflow Perm Buffer II (Cat. No. 558052), or BD™ Phosflow Perm Buffer III (Cat. No. 558050). Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system.

Flow cytometric analysis of PE Mouse anti-Sox2 in H9 cells. H9 human embryonic stem (ES) cells (WiCell, Madison, WI) were fixed (BD Cytofix™ buffer, Cat. No. 554655) for 10 minutes at 37°C, permeabilized with BD™ Phosflow Perm Buffer III (Cat. No. 558050) on ice for 30 minutes, and then stained with either PE Mouse anti-Sox2 (solid line) or PE Mouse IgG2a Isotype Control (dashed line).  Any of the three BD™ Phosflow permeabilization buffers may be used with this antibody: BD™ Phosflow Perm/Wash Buffer I (Cat. No. 557885), BD™ Phosflow Perm Buffer II (Cat. No. 558052), or BD™ Phosflow Perm Buffer III (Cat. No. 558050). Flow cytometry was performed on a BD FACSCanto™ II flow cytometry system.

Product Details
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BD Pharmingen™
ANOP3, MCOPS3, MGC2413
Human (QC Testing)
Mouse IgG2a
Human SOX2 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
20 µl
AB_1645334
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation.  Any of the three BD™ Phosflow permeabilization buffers may be used with this antibody: BD™ Phosflow Perm/Wash Buffer I (Cat. No. 557885), BD™ Phosflow Perm Buffer II (Cat. No. 558052), or BD™ Phosflow Perm Buffer III (Cat. No. 558050).

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
560291 Rev. 2
Antibody Details
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245610

The monoclonal antibody 245610 recognizes the Sox2 transcription factor.  Sox2  [SRY (sex determining region Y)-box 2] is a member of the  SRY-related HMG-box (SOX) family of transcription factors. Sox2 is required for the maintenance of the undifferentiated state of pluripotent stem cells.  Complexes of Sox2 with the homeobox transcription factors Oct3/4 and/or Nanog bind to the promoters of a network of genes that are involved in the maintenance of pluripotency and self renewal in stem cells. The 245610 antibody recognizes both human and mouse proteins.  Sox2 is also a marker of neural stem cells during embryonic development and in the adult brain.

560291 Rev. 2
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
560291 Rev.2
Citations & References
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Development References (3)

  1. Boyer LA, Lee TI, Cole MF, et al. Core transcriptional regulatory circuitry in human embryonic stem cells. Cell. 2005; 122:947-956. (Biology). View Reference
  2. Pan G, Thomson JA. Nanog and transcriptional networks in embryonic stem cell pluripotency. Cell Res. 2007; 17:42-49. (Biology). View Reference
  3. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006; 126(4):663-676. (Biology). View Reference
560291 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.