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PE Mouse Anti-Human Neuropilin-1 (CD304)
PE Mouse Anti-Human Neuropilin-1 (CD304)
Two-color flow cytometric analysis of Neuropilin-1 (CD304) expression on human peripheral blood lymphoid cells. Human peripheral blood mononuclear cells were stained with APC Mouse Anti-Human CD123 antibody (Cat. No. 560087) and either PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058; Left Plot) or PE Mouse Anti-Human Neuropilin-1 (CD304) antibody (Cat. No. 565951/565952; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD123 versus Neuropilin-1 (CD304) [or Ig Isotype control staining] were derived from gated events with the forward and side light-scatter characteristics of viable lymphoid cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two-color flow cytometric analysis of Neuropilin-1 (CD304) expression on human peripheral blood lymphoid cells. Human peripheral blood mononuclear cells were stained with APC Mouse Anti-Human CD123 antibody (Cat. No. 560087) and either PE Mouse IgG2b, κ Isotype Control (Cat. No. 555058; Left Plot) or PE Mouse Anti-Human Neuropilin-1 (CD304) antibody (Cat. No. 565951/565952; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD123 versus Neuropilin-1 (CD304) [or Ig Isotype control staining] were derived from gated events with the forward and side light-scatter characteristics of viable lymphoid cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
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BD Pharmingen™
Neuropilin; NRP; NP1; Blood dendritic cell antigen 4; BDCA-4; VEGF165R
Human (QC Testing)
Mouse IgG2b, κ
Recombinant Human Neuropilin-1
Flow cytometry (Routinely Tested)
5 µl
AB_2744361
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  6. An isotype control should be used at the same concentration as the antibody of interest.
565951 Rev. 1
Antibody Details
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U21-1283

The U21-1283 monoclonal antibody specifically recognizes Neuropilin-1 (NRP1), also known as CD304, Blood dendritic cell antigen 4 (BDCA4), and Vascular endothelial cell growth factor 165 receptor (VEGF165R). CD304 is a type I transmembrane glycoprotein involved in the development of the nervous and cardiovascular systems. It mediates the interaction, growth, survival, and migration of a variety of normal and tumor cells. CD304 is expressed on neurons, thymocytes, regulatory T cells, a subset of T follicular helper cells, dendritic cells, endothelial cells, and certain tumor cells. Neuropilin-1 has a very short cytoplasmic domain and interacts with various coreceptors to form ligand-binding, signal-transducing receptor complexes. CD304 complexes with Plexin-A family members to bind chemorepellent Class 3 Semaphorins and guide neuronal axon growth. It also functions as a coreceptor with VEGFR2/CD309 to stimulate angiogenesis in response to VEGF165. CD304 mediates the interactions between some T cells and dendritic cells.

        

565951 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
565951 Rev.1
Citations & References
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View product citations for antibody "565951" on CiteAb

Development References (6)

  1. Bruder D et al. Neuropilin-1: a surface marker of regulatory T cells.. Eur J Immunol. 2004; 34(3):623-630. (Biology). View Reference
  2. Dzionek A et al. BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood.. J Immunol. 2000; 165(11):6037-6046. (Biology). View Reference
  3. Mizui M, Kumanogoh A, Kikutani H. Immune semaphorins: novel features of neural guidance molecules.. J Clin Immunol. 2009; 29(1):1-11. (Biology). View Reference
  4. Pan Q et al. Neuropilin-1 binds to VEGF121 and regulates endothelial cell migration and sprouting.. J Biol Chem. 2007; 282(33):24049-24056. (Biology). View Reference
  5. Renand A, Milpied P, Rossignol J, et al. Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs.. PLoS ONE. 2013; 8(12):e85589. (Biology). View Reference
  6. Soker S, Takashima S, Miao HQ, Neufeld G, Klagsbrun M. Neuropilin-1 is expressed by endothelial and tumor cells as an isoform-specific receptor for vascular endothelial growth factor.. Cell. 1998; 92(6):735-45. (Biology). View Reference
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565951 Rev. 1

 

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.