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BD Horizon™ BV421 Rat Anti-Mouse Ig, κ Light Chain
Clone 187.1 (RUO)
Multicolor flow cytometric analysis of mouse Immunoglobulin κ Light Chain expression on mouse splenocytes. Splenic leucocytes from a BALB/c mouse were stained with APC Rat Anti-Mouse CD45R/B220 (Cat. No. 553092/561880) and with either a BD Horizon™ BV421 Rat IgG1, κ Isotype Control (Cat. No. 562868, Left Panel) or with the BD Horizon™ BV421 Rat Anti-Mouse Ig, κ Light Chain antibody (Cat. No. 562888, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of Ig, κ light chain (or Ig Isotype control staining) versus CD45R/B220 were derived from gated events with the light scattering characteristics of viable leucocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.
Multicolor flow cytometric analysis of mouse Immunoglobulin κ Light Chain expression on mouse splenocytes. Splenic leucocytes from a BALB/c mouse were stained with APC Rat Anti-Mouse CD45R/B220 (Cat. No. 553092/561880) and with either a BD Horizon™ BV421 Rat IgG1, κ Isotype Control (Cat. No. 562868, Left Panel) or with the BD Horizon™ BV421 Rat Anti-Mouse Ig, κ Light Chain antibody (Cat. No. 562888, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of Ig, κ light chain (or Ig Isotype control staining) versus CD45R/B220 were derived from gated events with the light scattering characteristics of viable leucocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.
Multicolor flow cytometric analysis of mouse Immunoglobulin κ Light Chain expression on mouse splenocytes. Splenic leucocytes from a BALB/c mouse were stained with APC Rat Anti-Mouse CD45R/B220 (Cat. No. 553092/561880) and with either a BD Horizon™ BV421 Rat IgG1, κ Isotype Control (Cat. No. 562868, Left Panel) or with the BD Horizon™ BV421 Rat Anti-Mouse Ig, κ Light Chain antibody (Cat. No. 562888, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of Ig, κ light chain (or Ig Isotype control staining) versus CD45R/B220 were derived from gated events with the light scattering characteristics of viable leucocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.
Multicolor flow cytometric analysis of mouse Immunoglobulin κ Light Chain expression on mouse splenocytes. Splenic leucocytes from a BALB/c mouse were stained with APC Rat Anti-Mouse CD45R/B220 (Cat. No. 553092/561880) and with either a BD Horizon™ BV421 Rat IgG1, κ Isotype Control (Cat. No. 562868, Left Panel) or with the BD Horizon™ BV421 Rat Anti-Mouse Ig, κ Light Chain antibody (Cat. No. 562888, Right Panel). Two-color flow cytometric dot plots showing the correlated expression of Ig, κ light chain (or Ig Isotype control staining) versus CD45R/B220 were derived from gated events with the light scattering characteristics of viable leucocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.
Product Details
BD Horizon™
Ig kappa chain C region; IGKC; Igk-C; Ig, κ
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG1, κ
Mouse IgG2b κ secreted by MPC-11 plasmacytoma
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2737867
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- Brilliant Violet™ 421 is a trademark of Sirigen.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
BV421 Rat IgG1, κ Isotype Control RUO
Size
50 µg
Cat No.
562868
APC Rat Anti-Mouse CD45R/B220 RUO
Size
0.1 mg
Cat No.
553092
APC Rat Anti-Mouse CD45R/B220 RUO
Size
25 mg
Cat No.
561880
562888 Rev. 1
Antibody Details
187.1
The 187.1 monoclonal antibody specifically binds to kappa light chains of mouse immunoglobulins. The 187.1 antibody does not react with mouse λ1 or λ2 immunoglobulin lights chains or mouse immunoglobulin heavy chains.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.
562888 Rev. 1
Format Details
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
407 nm
423 nm
562888 Rev.1
Citations & References
View product citations for antibody "562888" on CiteAb
Development References (1)
-
Yelton DE, Desaymard C, Scharff MD. Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies. Hybridoma. 1981; 1(1):5-11. (Immunogen). View Reference
562888 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.