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BD Horizon™ BV421 Mouse Anti-Human CD340 (HER-2)

Name: BV421 Mouse Anti-Human CD340 (HER-2)
Brand: BV421 Mouse Anti-Human CD340 (HER-2)
SKU: 566459

Clone 24D2 (RUO)

Flow cytometric analysis of CD340 (HER-2) expression on human breast cancer cell lines. Cells from either the MCF7 (ATCC HTB-22; Left Panel) or SK-BR-3 (ATCC HTB-30D-2; Right Panel) human breast adenocarcinoma cell line were stained with either BD Horizon BV421™ Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histograms) or BD Horizon BV421 Mouse Anti-Human CD340 (HER-2) antibody (Cat. No. 566458/566459; solid line histograms) at 0.5 μg/test. The histograms showing CD340 (HER-2) expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.

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Product Details

Brand: BD Horizon™

Alternative Name: ERBB2; erbB-2; c-erb B2; HER-2/neu; Neu; Herstatin; MLN 19; NGL; TKR1

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: NIH-3T3 transfected with Human HER-2

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Workshop Number: HCDM 2006

RRID: AB_2744366

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349).

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (BSA) RUO

Size 500 ML Cat No. 554657

Stain Buffer (FBS) RUO

Size 500 ML Cat No. 554656

Brilliant Stain Buffer RUO

Size 100 Tests Cat No. 563794

Brilliant Stain Buffer RUO

Size 1000 Tests Cat No. 566349

BV421 Mouse IgG1, k Isotype Control RUO

Size 50 µg Cat No. 562438

BV421 Mouse Anti-Human CD340 (HER-2) RUO

Size 0.1 µg Cat No. 566458

View Details

566459 Rev. 1

Antibody Details

24D2

The 24D2 monoclonal antibody specifically recognizes CD340 which is also known as Human epidermal growth factor receptor 2 (HER-2 or HER2), Neu (neuro/glioblastoma derived oncogene homolog), or Metastatic lymph node gene 19 protein (MLN19). CD340 (HER-2) is a ~185 kDa type I transmembrane glycoprotein that is encoded by ERBB2 (erb-b2 receptor tyrosine kinase 2) and belongs to the ERBB family of receptor tyrosine kinases that includes EGFR, HER3, and HER4. Although CD340 (HER-2) has no identified direct-binding ligand, it can form heterodimers with other ligand-bound ERBB family members. CD340 (HER-2) helps activate intracellular signaling pathways including mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3 kinase (PI-3K) through its cytoplasmic tyrosine kinase-containing region. CD340 (HER-2) is expressed by bone marrow mesenchymal stem cells. It is overexpressed and plays a significant role in the development of multiple human cancers including some leukemias, neuroblastomas, and carcinomas of the breast, ovary, cervix, colon, and lung.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

566459 Rev. 1

Format Details

BV421

The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: BV421

Excitation Max: 407 nm

Emission Max: 423 nm

566459 Rev.1

Citations & References

View product citations for antibody "566459" on CiteAb

Development References (4)

Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007; 1106:262-271. (Clone-specific: Flow cytometry). View Reference
Bühring HJ, Sures I, Jallal B, et al. The receptor tyrosine kinase p185HER2 is expressed on a subset of B-lymphoid blasts from patients with acute lymphoblastic leukemia and chronic myelogenous leukemia.. Blood. 1995; 86(5):1916-23. (Immunogen: Flow cytometry). View Reference
Yan M, Schwaederle M, Arguello D, Millis SZ, Gatalica Z, Kurzrock R. HER2 expression status in diverse cancers: review of results from 37,992 patients.. Cancer Metastasis Rev. 2015; 34(1):157-64. (Biology). View Reference
Zola H, Swart B, Banham A, et al. CD molecules 2006--human cell differentiation molecules.. J Immunol Methods. 2007; 319(1-2):1-5. (Clone-specific: Flow cytometry). View Reference

View All (4)

566459 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.