The JOVI.3 monoclonal antibody specifically recognizes the human variable beta 3 region of the T cell receptor for antigen (TCR Vβ3). The JOVI.3 antibody reportedly stains approximately 1-10% of human CD3-positive peripheral blood lymphocytes including CD4+ or CD8+ T cells. The variation in human TCR Vβ3 expression correlates with allelic polymorphism in the spacer region of recombination signal sequence. The JOVI.3 antibody is mitogenic for peripheral blood T cells expressing TCR Vβ3.
The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.