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Multiparameter flow cytometric analysis of IL-10Rβ (CD210b) expression on human peripheral blood leucocytes. Human whole blood was stained with PE Mouse Anti-Human HLA-DR (Cat. No. 555812/556644/560943) and FITC Mouse Anti-Human CD14 (Cat. No. 555397/557153/561712) antibodies and either Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat. No. 557714) or Alexa Fluor® 647 Mouse Anti-Human IL-10Rβ (CD210b) antibody (Cat. No. 564372). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System. Panel 1. Two-parameter contour plots show the correlated expression of isotype control (Left Plot) or IL-10Rβ (CD210b) (Right Plot) versus side-light scatter (SSC-A) characteristics for gated events with the forward light scatter properties of total viable leucocytes. Panel 2. For gated events with the forward and side-light scatter characteristics of viable mononuclear leucocytes, the two-color contour plots show the correlated expression of CD14 (Left Plot) or IL-10Rβ (CD210b) (Right Plot) versus HLA-DR for HLA-DR+ (and HLA-DR-) lymphocytes and CD14+HLA-DR+ monocytes.


BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human IL-10Rβ (CD210b)

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Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
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Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The 90220 monoclonal antibody specifically binds to the beta subunit (IL-10R beta/IL-10RB/IL-10Rβ) of the heteromeric IL-10 Receptor complex. This type I transmembrane glycoprotein is also known as, subunit 2 (IL-10R2) or CD210b. The IL-10 Receptor complex is formed by the binding of dimeric IL-10 to two cell surface alpha subunits of the IL-10 Receptor (CD210a/IL-10RA/IL-10Rα). This complex can then recruit two CD210b subunits resulting in the transduction of intracellular JAK/STAT pathway signals. CD210b is also a component of cytokine receptor complexes specific for IL-22, IL-26, IL-28 and IL-29. CD210b is expressed by a variety of cell types including T cells, B cells, NK cells, monocytes and macrophages.
Development References (5)
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Crepaldi L, Gasperini S, Lapinet JA, et al. Up-regulation of IL-10R1 expression is required to render human neutrophils fully responsive to IL-10.. J Immunol. 2001; 167(4):2312-22. (Biology). View Reference
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Donnelly RP, Sheikh F, Kotenko SV, Dickensheets H. The expanded family of class II cytokines that share the IL-10 receptor-2 (IL-10R2) chain. J Leukoc Biol. 2004; 76(2):314-321. (Biology). View Reference
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Koppelman B, Neefjes JJ, de Vries JE, de Waal Malefyt R. Interleukin-10 down-regulates MHC class II alphabeta peptide complexes at the plasma membrane of monocytes by affecting arrival and recycling. Immunity. 1997; (6):861-871. (Biology). View Reference
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Kotenko SV, Krause CD, Izotova LS, Pollack BP, Wu W, Pestka S. Identification and functional characterization of a second chain of the interleukin-10 receptor complex. EMBO J. 1997; 16(19):5894-5903. (Biology). View Reference
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Liu Y, Wei SH, Ho AS, de Waal Malefyt R, Moore KW. Expression cloning and characterization of a human IL-10 receptor. J Immunol. 1995; 152(4):1821-1829. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.